The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
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DOI：
10.1371/journal.pone.0158700
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Angrand PO
中科院分区：
文献类型：
--
作者：
Dupret B;Völkel P;Le Bourhis X;Angrand PO
Polycomb Repressive Complex（PRC）1通过染色质结构重组调控基因表达程序。通过相互排斥，不同的PCGF成员产生各种PRC 1复合物，具有潜在的不同细胞功能。在这种情况下，每个PCGF家族成员的分子功能仍然难以捉摸。PCGF家族成员在斑马鱼发育和尾鳍再生过程中表达的研究表明，斑马鱼PCGF基因受到不同的调控，并且Pcgf亚基组成的所有PRC 1复合物并不总是存在于相同的组织中。为了揭示Pcgf 1在斑马鱼中的功能，使用TALEN技术产生突变系。突变pcgf 1-/-鱼是可行的和肥沃的，但在早期发育阶段的生长速度降低，在pcgf 1基因功能的情况下，大量的pcgf 1-/-鱼显示出过早衰老的迹象。第一个缺乏Pcgf 1功能的脊椎动物模型表明，这种Polycomb Group蛋白参与早期胚胎发生期间的细胞增殖，并建立了表观遗传学和衰老之间的联系。
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of each of the PCGF family members remains elusive. The study of PCGF family member expression in zebrafish development and during caudal fin regeneration reveals that the zebrafish pcgf genes are subjected to different regulations and that all PRC1 complexes in terms of Pcgf subunit composition are not always present in the same tissues. To unveil the function of Pcgf1 in zebrafish, a mutant line was generated using the TALEN technology. Mutant pcgf1-/- fish are viable and fertile, but the growth rate at early developmental stages is reduced in absence of pcgf1 gene function and a significant number of pcgf1-/- fish show signs of premature aging. This first vertebrate model lacking Pcgf1 function shows that this Polycomb Group protein is involved in cell proliferation during early embryogenesis and establishes a link between epigenetics and aging.