SFRP5 acts as a mature adipocyte marker but not as a regulator in adipogenesis
SFRP5 acts as a mature adipocyte marker but not as a regulator in adipogenesis
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SFRP5 作为成熟脂肪细胞标记物，但不作为脂肪生成的调节剂。
DOI：
10.1530/jme-14-0037
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发表时间：
2014-12-01
影响因子：
3.5
通讯作者：
Ning, Guang
中科院分区：
文献类型：
--
作者：
Wang, Rui;Hong, Jie;Ning, Guang
WNT/β-连环蛋白信号传导参与调节脂肪形成，并且其失调发生在肥胖症中。分泌型卷曲相关蛋白5（SFRP 5）是一种WNT蛋白抑制剂;然而，其在脂肪形成和肥胖中的作用是有争议的。在这项研究中，我们观察到SFRP 5 mRNA水平在肥胖人类和小鼠的脂肪组织中增加。Sfrp 5的表达在白色和棕色脂肪细胞的分化过程中逐渐被诱导，并且在成熟脂肪细胞而不是前脂肪细胞中高度增加。然而，Sfrp 5的外源性过表达的影响表明，Sfrp 5可能不直接调节脂肪形成在体外研究的条件下。此外，SFRP 5不抑制前脂肪细胞中的经典WNT/β-连环蛋白信号通路。随后，我们使用ELISA测量了肥胖患者和非肥胖受试者的循环SFRP 5水平，没有发现任何显著差异。总的来说，这些发现表明Sfrp 5代表了成熟脂肪细胞标记基因的候选者。我们的数据为SFRP 5在白色和棕色脂肪细胞的脂肪形成和肥胖中的作用提供了新的证据。
WNT/beta-catenin signalling is involved in regulating adipogenesis, and its dysregulation occurs in obesity. Secreted frizzled-related protein 5 (SFRP5) is a WNT protein inhibitor; however, its role in adipogenesis and obesity is controversial. In this study, we observed that SFRP5 mRNA levels were increased in the fat tissues of obese humans and mice. Sfrp5 expression was gradually induced during differentiation of white and brown adipocytes and was highly increased in mature adipocytes rather than preadipocytes. However, the effects of the exogenous overexpression of Sfrp5 indicated that Sfrp5 may not directly regulate adipogenesis in vitro under the conditions studied. Moreover, SFRP5 did not inhibit the canonical WNT/beta-catenin signalling pathway in preadipocytes. Subsequently, we measured the levels of circulating SFRP5 in obese patients and non-obese subjects using ELISA and did not find any significant difference. Collectively, these findings indicate that Sfrp5 represents a candidate for a mature adipocyte marker gene. Our data provide new evidence concerning the role of SFRP5 in adipogenesis of white and brown adipocytes and obesity.