Cellular expression of α7 nicotinic acetylcholine receptor protein in the temporal cortex in Alzheimer's and Parkinson's disease -: A stereological approach
Cellular expression of α7 nicotinic acetylcholine receptor protein in the temporal cortex in Alzheimer's and Parkinson's disease -: A stereological approach
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DOI：
10.1006/nbdi.2000.0317
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发表时间：
2000-12-01
影响因子：
6.1
通讯作者：
Schröder, H
中科院分区：
文献类型：
--
作者：
Banerjee, C;Nyengaard, JR;Schröder, H
阿尔茨海默病和帕金森病的认知缺陷与胆碱能传递紊乱密切相关。烟碱乙酰胆碱受体蛋白的减少已通过蛋白质印迹法和免疫组织化学进行了评估。然而，体视学尚未被用于评估表达受体的人类大脑皮层神经元的数量。我们的方法应用了α 7亚基免疫组化与体视学技术的组合，使用定义的软脑膜表面延伸作为参考标准。阿尔茨海默病患者颞叶皮质中表达α 7亚单位蛋白的神经元数量约为对照组的一半，而帕金森病患者的数量介于两者之间。神经元总数无差异。这些结果证实了对阿尔茨海默病皮层的非体视学研究，并首次显示了帕金森病受体表达的类似减少。他们提供的证据表明，不仅阿尔茨海默氏痴呆症，而且帕金森氏病的认知缺陷可能与烟碱受体表达减少有关。(C)北京大学出版社.
Cognitive deficits in Alzheimer's and Parkinson's disease are closely related to disturbed cholinergic transmission. The decrease of nicotinic acetylcholine receptor protein has been assessed by Western blotting and immunohistochemistry. Stereology, however, has not been used to assess numbers of receptor-expressing human cerebrocortical neurons. Our approach applies a combination of alpha7 subunit-immunohistochemistry with a stereological technique using defined stretches of pial surface as reference standard. The number of alpha7 subunit protein-expressing neurons in the Alzheimer temporal cortices amounted to approximately half of that of controls while numbers in Parkinson patients lay in between. No differences in the total number of neurons were seen. These results corroborate nonstereological studies on Alzheimer cortices and for the first time show a similar decrease in receptor expression in Parkinson's disease. They provide evidence that not only Alzheimer dementia but also cognitive deficits in Parkinson's disease may be related to decreased nicotinic receptor expression. (C) 2000 Academic Press.