Dietary alpha-tocopherol supplementation on antioxidant defenses after in vivo iron overload in rats
Dietary alpha-tocopherol supplementation on antioxidant defenses after in vivo iron overload in rats
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DOI：
10.1016/s0300-483x(97)00135-2
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发表时间：
1997-12-19
期刊：
影响因子：
4.5
通讯作者：
Puntarulo, S
中科院分区：
文献类型：
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作者：
Galleano, M;Puntarulo, S
研究了膳食α-生育酚（α-T）补充剂对铁超负荷依赖性氧化损伤的影响。向雄性Wistar大鼠饲喂补充有2.5%羰基铁和/或200 mg/kg α-生育酚乙酸酯的饲料6周。脂质和蛋白质的氧化增加了大鼠肝脏中的铁超载，和α-T的膳食补充剂有效地防止这些影响。铁超载降低，过氧化氢酶和锰超氧化物歧化酶活性分别为49%和54%，谷胱甘肽过氧化物酶活性没有影响。α-T补充没有阻止过氧化氢酶和锰超氧化物歧化酶活性的抑制。铁膳食过量对肝脏α-T和泛醇9（UQ 9）含量没有影响。铁超载后，全肝和肝线粒体中的UQ 10含量分别下降了58%和54%。与对照值相比，α-T补充导致肝脏中α-T、UQ 9和UQ 10含量显著增加，并部分防止了由于铁过量导致的UQ 10含量降低。这里提出的结果表明，铁过载的初始阶段导致肝脏氧化损伤（在脂质和蛋白质氧化方面进行评估），抗氧化防御能力下降。补充α-T影响了肝脏中脂溶性抗氧化剂的含量，这表明在细胞水平上协调的抗氧化反应调节了过量铁可用性的影响。(C)1997 Elsevier Science爱尔兰有限公司
The effect of dietary alpha-tocopherol (alpha-T) supplementation on iron overload-dependent oxidative damage was studied. Male Wistar rats were fed diets supplemented with 2.5% carbonyl iron and/or 200 mg/kg of alpha-tocopheryl acetate for 6 weeks. Oxidation of lipids and proteins were increased by iron overload in rat liver, and alpha-T dietary supplementation effectively prevented these effects. Iron overload decreased both, catalase and Mn-superoxide dismutase activities by 49 and 54%, respectively, with no effect on glutathione peroxidase activity. alpha-T supplementation did not prevent the inhibition measured in catalase and Mn-superoxide dismutase activities. Iron dietary excess had no effect on liver alpha-T and ubiquinol 9 (UQ9) content. Ubiquinol 10 (UQ10) content after iron overload was decreased by 58 and 54% in whole liver and liver mitochondria, respectively. alpha-T supplementation led to significant increases in alpha-T, UQ9 and UQ10 content in liver, as compared to control values, and partially prevented the decrease in UQ10 content due to iron excess. The results presented here indicate that initial stages of iron overload led to oxidative damage in liver (evaluated in terms of lipid and protein oxidation) with a decline in antioxidant defenses. alpha-T supplementation affected the liver content of lipid soluble antioxidants, suggesting a concerted antioxidant response at the cellular level to modulate the effect of excess iron availability. (C) 1997 Elsevier Science Ireland Ltd.