Laser nephelometry and radial immunodiffusion compared for immunoglobulin quantification in pathological sera.
Laser nephelometry and radial immunodiffusion compared for immunoglobulin quantification in pathological sera.
复制标题
激光比浊法和径向免疫扩散法对病理血清中免疫球蛋白的定量进行了比较。
DOI：
10.1093/clinchem/28.1.180
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发表时间：
1982
影响因子：
9.3
通讯作者：
R. Creyssel
中科院分区：
文献类型：
--
作者：
H. Cloppet;A. Francina;H. Coquelin;Y. Boucaud;P. Hutinel;R. Creyssel
我们评估了Behring、Hyland和Beckman的散射比浊计对单克隆性免疫球蛋白病患者血清中的免疫球蛋白、免疫球蛋白A和免疫球蛋白M的定量。对每种免疫球蛋白类别和同一免疫球蛋白的不同浓度的每种仪器的批内精密度与径向免疫扩散法进行了比较。没有一个浊度计显示出明显更高的精确度。三种浊度计与醋酸纤维素电泳法的相关性均较好。径向免疫扩散法测得的平均值高于散射比浊法的测定值。
We evaluated nephelometers from Behring, Hyland, and Beckman for IgG, IgA, and IgM quantitation in sera from patients with monoclonal gammopathies. The intra-batch precision of each instrument for each immunoglobulin class and for different concentrations of the same immunoglobulin was compared to the one obtained with the radial immunodiffusion method. No nephelometer showed a clearly better precision. The correlation with cellulose acetate electrophoresis was good for each of the three nephelometers. The mean value by the radial immunodiffusion method was higher than corresponding determinations by nephelometry.