Routine use of the gen-probe MTD2 amplification test for detection of Mycobacterium tuberculosis in clinical specimens in a large public health mycobacteriology laboratory
Routine use of the gen-probe MTD2 amplification test for detection of Mycobacterium tuberculosis in clinical specimens in a large public health mycobacteriology laboratory
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DOI：
10.1016/s0732-8893(99)00086-3
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发表时间：
1999-11-01
影响因子：
2.9
通讯作者：
Jamieson, FB
中科院分区：
文献类型：
--
作者：
Chedore, P;Jamieson, FB
MEW版本的扩增结核分枝杆菌直接测试，MTD 2（Gen-Probe Inc.，San Diego，CA）作为常规检测算法的一部分，在我们实验室的选定呼吸道和非呼吸道标本中检测结核分枝杆菌（MTB）。在安大略埃托比科克的中央公共卫生实验室，我们接收来自安大略省所有地区的用于检测分枝杆菌的标本。该实验室每年处理约25，000份标本，并接收约2000份参考培养物进行鉴定。每年有600至700例新的肺结核病例。1997 ~ 1998年共检测823例标本，其结果与放射性培养（Bactec，Becton Dickinson，Sparks，MD）和临床诊断结果进行比较，总的敏感性、特异性、阳性预测值和阴性预测值分别为100%、99.6%、97.4%和100%。202例呼吸道结核和56例肺外结核病例在标本到达实验室后0-4天内被MTD 2检测到。通过选择合适的标本进行检测，MTD 2可以为MTB bz临床标本的检测提供一种快速、准确和经济有效的方法。(C)1999 Elsevier Science Inc.
The MEW version of the Amplified Mycobacterium Tuberculosis Direct Test, MTD2 (Gen-Probe Inc., San Diego, CA) has been implemented as part of the regular testing algorithm for defecting Mycobacterium tuberculosis (MTB) in selected respiratory and non-respiratory specimens in our laboratory. At the Central Public Health Laboratory, Etobicoke, Ontario, we receive specimens for the detection of mycobacteria from all areas of the Province of Ontario. The laboratory processes approximately 25,000 specimens per year, and receives approximately 2000 reference cultures for identification. There are 600 to 700 new cases of tuberculosis defected yearly. Over the 1-year period (1997-98), 823 specimens Were tested by MTD2 and the results were compared with radiometric culture (Bactec, Becton Dickinson, Sparks, MD) and clinical diagnosis, giving an overall sensitivity, specificity, positive predictive value and negative predictive values of 100%, 99.6%, 97.4% and 100%, respectively. Two hundred and two cases of respiratory TB and 56 cases of extrapulmonary TB were detected by MTD2 within 0-4 days of specimen arrival in the laboratory. By appropriate selection of specimens for testing, the MTD2 can provide a fast, accurate, and cost-effective method for the detection of MTB bz clinical specimens. (C) 1999 Elsevier Science Inc.