PROLONGED ISCHEMIA INCREASES SEVERITY OF REJECTION IN SKIN FLAP ALLOTRANSPLANTATION IN RATS
PROLONGED ISCHEMIA INCREASES SEVERITY OF REJECTION IN SKIN FLAP ALLOTRANSPLANTATION IN RATS
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DOI：
10.1002/micr.20728
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发表时间：
2010-01-01
期刊：
影响因子：
2.1
通讯作者：
Wei, Fu-Chan
中科院分区：
文献类型：
--
作者：
Shimizu, Fumiaki;Okamoto, Osamu;Wei, Fu-Chan
为了研究缺血时间与同种异体移植排斥反应的关系，我们将褐挪威大鼠腹股沟带血管皮瓣移植到Lewis大鼠身上。缺血时间分别为1小时和6小时进行比较。采用环孢素A作为免疫抑制剂。结果表明，体内缺血6小时组的排斥反应更为严重，体外混合淋巴细胞反应检测也显示，缺血6小时组的抗供体反应比缺血1小时组更强烈。免疫组化研究显示，缺血6小时组MHCⅱ类抗原表达高于缺血1小时组。这些结果表明，较长的缺血时间比较短的缺血时间通过上调MHC II类抗原诱导对同种异体移植组织更严重的排斥反应。这些发现有望为进一步研究同种异体移植排斥反应机制提供理论依据。(C) 2010 Wiley-Liss, Inc。显微外科杂志，2010。
To investigate the relationship between ischemic time and rejection against allotransplants, vascularized cutaneous flaps from the groin of Brown Norway rats were transplanted to Lewis rats. The ischemic time was set at 1 hour and 6 hours for comparison. Cycrosporine A was used as the immunosuppressant. The results showed more severe rejection in the 6 hours ischemic time group in vivo, and in vitro examination using mixed lymphocyte reaction assay also demonstrated a greater antidonor response in 6 hours-ischemic group than that in 1 hour-group. Immunohistochemical study demonstrated more MHC class II antigen expression in 6 hours-ischemic group than in 1 hour-group. These results suggest that longer ischemic time induces more severe rejection against allo-transplanted tissue compared with the shorter one through an upregulation of MHC class II antigen. It is expected that these findings contribute to the studies for investigating the mechanism of rejection against the allo-transplants. (C) 2010 Wiley-Liss, Inc. Microsurgery 30:132-137, 2010.