A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana (Publication with Expression of Concern)
A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana (Publication with Expression of Concern)
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DOI：
10.1016/s0092-8674(00)00095-7
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发表时间：
2000-09-29
期刊：
影响因子：
64.5
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
--
作者：
Voinnet, O;Lederer, C;Baulcombe, DC
在植物中，病毒诱导RNA介导的防御，类似于转基因的转录后基因沉默（PTGS）。在这里，我们证明与马铃薯X病毒（PVX）的PTGS作为一个系统的，序列特异性防御系统。然而，在移植实验或运动缺陷形式的PVX，我们不能检测到系统沉默，除非25 kDa的病毒运动蛋白（p25）是无功能的。对p25的研究揭示了PTGS途径的两个分支，其在对应于靶RNA的25个核苷酸RNA的产生中会聚。其中一个分支是病毒诱导的PTGS所特有的，不受p25的影响。第二个分支是病毒和转基因诱导的PTGS共有的，被p25阻断，并且可能产生系统性沉默信号。
In plants, viruses induce an RNA-mediated defense that is similar to posttranscriptional gene silencing (PTGS) of transgenes. Here we demonstrate with potato virus X (PVX) that PTGS operates as a systemic, sequence-specific defense system. However, in grafting experiments or with movement defective forms of PVX, we could not detect systemic silencing unless the 25 kDa viral movement protein (p25) was made nonfunctional. Investigation of p25 revealed two branches to the PTGS pathway that converge in the production of 25 nucleotide RNAs corresponding to the target RNA. One of these branches is unique to virus-induced PTGS and is not affected by p25. The second branch is common to both virus- and transgene-induced PTGS, is blocked by p25, and is likely to generate the systemic silencing signal.