Gene expression analysis of four WIR1-like genes in floret tissues of European winter wheat after challenge with G-zeae
Gene expression analysis of four WIR1-like genes in floret tissues of European winter wheat after challenge with G-zeae
复制标题
DOI：
10.1007/s10681-011-0498-7
复制
发表时间：
2012-07-01
期刊：
影响因子：
1.9
通讯作者：
Schweizer, G.
中科院分区：
文献类型：
--
作者：
Diethelm, M.;Rhiel, M.;Schweizer, G.
小麦赤霉病是一种危害小麦和其他谷物的极具破坏性的病害，对粮食造成严重的真菌毒素污染。一些分子作图研究导致检测到对欧洲冬小麦赤霉病抗性有轻微到中等影响的QTL。参与这些基因型防御反应的基因在很大程度上仍不清楚。WIR1(小麦诱导抗性1)基因在各种真菌病原菌侵袭过程中在谷类中表达上调，但其在抗性中的作用尚不明确。本研究对3个WIR1基因和1个与WIR1高度相似的基因在接种赤霉菌后在欧洲冬小麦中的表达进行了研究。用玉米赤霉菌分生孢子或水作为对照，对4个冬小麦品种(Dream、Lynx、G16-92、Hussar)的小花组织进行侵染处理，接种后0~96h内取样6次。实时荧光定量聚合酶链式反应显示，这四种基因在抗性品种中的表达水平均高于敏感品种。在G16-92/Hussar作图群体中，WIR1b和一个与WIR1基因序列相似的基因被定位在染色体5DS上。在Dream/Lynx作图群体中，两个标记为WIR1a的基因被定位在7BS染色体上的抗赤霉病QTL区间。这些基因可能是赤霉病数量抗性的候选基因。
Fusarium head blight (FHB) is a highly destructive disease of wheat and other cereals which causes serious mycotoxin contaminations of grain. A number of molecular mapping studies led to the detection of QTL with small to moderate effects on FHB resistance in European winter wheat. Genes involved in the defence reaction of these genotypes remain largely unknown. WIR1 (wheat induced resistance 1) genes have been shown to be upregulated in cereals during attack of various fungal pathogens; however, their role in resistance is ambiguous. In this study, the expression of three WIR1 genes and a gene with high sequence similarity to WIR1 was investigated in European winter wheat genotypes after inoculation with Giberella zeae. Floret tissues of four winter wheat genotypes (Dream, Lynx, G16-92, Hussar) were challenged with G. zeae conidia or water (control) and sampled six times during 0-96 h after inoculation. Quantitative real-time PCR showed that all four genes were highly upregulated in the resistant genotypes compared to the susceptible ones. WIR1b and a gene with sequence similarity to WIR1 genes mapped to chromosome 5DS in the G16-92/Hussar mapping population. Two genes annotated as WIR1a mapped in the interval of a FHB resistance QTL on chromosome 7BS in the Dream/Lynx mapping population. These could be considered possible candidate genes for quantitative FHB resistance.