Ventrally emigrating neural tube cells contribute to the normal development of heart and great vessels
Ventrally emigrating neural tube cells contribute to the normal development of heart and great vessels
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DOI：
10.1016/s1537-1891(03)00003-x
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发表时间：
2003-02-01
影响因子：
4
通讯作者：
Sohal, GS
中科院分区：
文献类型：
--
作者：
Ali, MM;Farooqui, FA;Sohal, GS
我们调查了最近描述的神经管细胞群，它参与了各种组织的发育，对心脏和大血管的发育做出了贡献。这些细胞被称为腹侧迁徙神经管(Vent)细胞，起源于后脑神经管的腹侧部分，在脑神经连接处迁徙，并分布于各自的靶组织。在鸡胚胎中，位于迷走神经水平的尾侧后脑神经管的气孔细胞被标记了含有LacZ基因的复制缺陷逆转录病毒，这些细胞在该区域的神经峰迁移后被标记。在较老的胚胎中，在包括脑室、心房、其隔膜、主肺间隔和心脏大血管在内的各种位置都检测到了气孔细胞。免疫组织化学染色显示，通气孔细胞分化为大血管的平滑肌细胞。在此之前，已有向心肌细胞分化的报道。在离开神经管之前摘除通气孔细胞会导致一些常见的心血管畸形：薄壁的室壁和心房、室间隔和房间隔缺损、永存的动脉干和大血管狭窄。这些结果表明，一种新的神经管细胞群也有助于心脏和大血管的正常发育。因此，心脏和大血管由三种来源的细胞发育而来：中胚层细胞、神经脊细胞和喷口细胞。(C)2003 Elsevier Science Inc.保留所有权利。
We investigated the contributions of a recently described population of neural tube cells, which participates in the development of a variety of tissues, to the development of the heart and great vessels. These cells, termed as the ventrally emigrating neural tube (VENT) cells, originate in the ventral part of the hindbrain neural tube, emigrate at the site of attachment of the cranial nerves, and populate their respective target tissues. VENT cells of the caudal hindbrain neural tube at the level of the vagus nerve, which were previously reported to migrate into the heart, were tagged with replication-deficient retroviruses containing the LacZ gene in chick embryos, after the emigration of neural crest from this region. In older embryos, VENT cells were detected in a variety of locations including the ventricles, atria, their septa, aorticopulmonary septum, and great vessels of the heart. Immunostaining with a specific marker revealed that VENT cells differentiated into smooth muscle cells of great vessels. Differentiation of VENT cells into cardiac muscle cells was reported previously. Extirpation of the VENT cells prior to their departure from the neural tube resulted in some common cardiovascular malformations: thin-walled ventricles and atria, ventricular and atrial septal defects, persistent truncus arteriosus, and stenosis of the great vessels. These results suggest that a novel population of neural tube cells also contributes to the normal development of the heart and great vessels. Thus, the heart and great vessels develop from three sources of cells: mesoderm, neural crest, and VENT cells. (C) 2003 Elsevier Science Inc. All rights reserved.