Reference-Free Traction Force Microscopy Platform Fabricated via Two-Photon Laser Scanning Lithography Enables Facile Measurement of Cell-Generated Forces.
Reference-Free Traction Force Microscopy Platform Fabricated via Two-Photon Laser Scanning Lithography Enables Facile Measurement of Cell-Generated Forces.
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DOI：
10.1021/acsami.9b04362
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发表时间：
2019-05-22
影响因子：
9.5
通讯作者：
Slater JH
中科院分区：
文献类型：
--
作者：
Banda OA;Sabanayagam CR;Slater JH
细胞通过细胞骨架收缩机械地探测周围环境，从而感知微环境的物理性质并做出反应。材料对这些应力的响应可以通过牵引力显微镜 (TFM) 进行测量。传统的 TFM 平台存在一些局限性，包括可变的空间分辨率、难以获得完整的三维 (3D) 变形/应力分布，以及需要移除或松弛被测量的细胞以确定零应力状态。为了克服这些限制，我们开发了一种双光子光化学耦合方法来制造一个新的 TFM 平台，该平台可以对荧光基准标记的 3D 放置进行高分辨率控制，以便轻松测量细胞产生的剪切力和牵引力的法向分量。 3D 标记阵列的高度受控放置提供了内置的零应力状态，无需干扰正在测量的细胞，同时还提供了更高的吞吐量。使用这个平台，我们发现细胞产生的剪切力和法向力分量的大小在空间和时间上都是相关的。这个新平台提供的测量细胞产生的力的简便性和增加的吞吐量将对机械转导界和其他人有用。
Cells sense and respond to the physical nature of their microenvironment by mechanically probing their surroundings via cytoskeletal contractions. The material response to these stresses can be measured via traction force microscopy (TFM). Traditional TFM platforms present several limitations including variable spatial resolution, difficulty in attaining the full three-dimensional (3D) deformation/stress profile, and the requirement to remove or relax the cells being measured to determine the zero-stress state. To overcome these limitations, we developed a two-photon, photochemical coupling approach to fabricate a new TFM platform that provides high-resolution control over the 3D placement of fluorescent fiducial markers for facile measurement of cell-generated shear and normal components of traction forces. The highly controlled placement of the 3D marker array provides a built-in, zero stress state eliminating the need to perturb the cells being measured while also providing increased throughput. Using this platform, we discovered that the magnitude of cell-generated shear and normal force components are linked both spatially and temporally. The facile nature and increased throughput of measuring cell-generated forces afforded by this new platform will be useful to the mechanotransduction community and others.
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影响因子：
3.3
作者：
Panagiotakopoulou M;Lendenmann T;Pramotton FM;Giampietro C;Stefopoulos G;Poulikakos D;Ferrari A
通讯作者：
Ferrari A
影响因子：
10
作者：
Heintz, Keely A.;Bregenzer, Michael E.;Mantle, Jennifer L.;Lee, Kelvin H.;West, Jennifer L.;Slater, John H.
通讯作者：
Slater, John H.
影响因子：
41.2
作者：
Luo, Y;Shoichet, MS
通讯作者：
Shoichet, MS
影响因子：
14
作者：
Kim, Kyuri;Kim, Keumyeon;Lee, Haeshin
通讯作者：
Lee, Haeshin
影响因子：
7.5
作者：
Lv H;Li L;Sun M;Zhang Y;Chen L;Rong Y;Li Y
通讯作者：
Li Y