Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice.
Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice.
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DOI：
10.1186/1477-3155-8-16
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发表时间：
2010-07-14
影响因子：
10.2
通讯作者：
Gurunathan S
中科院分区：
文献类型：
--
作者：
Barathmanikanth S;Kalishwaralal K;Sriram M;Pandian SR;Youn HS;Eom S;Gurunathan S
氧化应激对糖尿病并发症的发病至关重要，其中高血糖引起的异常代谢环境导致多种并发症的发生。一种能够抑制氧化应激介导的糖尿病进展的生物抗氧化剂；在高血糖期间仍然是时代的需要。本研究旨在研究生物合成金纳米颗粒（AuNPs）对链脲佐菌素诱导的糖尿病小鼠高血糖状态的控制作用。AuNPs通过抑制高血糖时脂质过氧化和ROS的生成，将糖尿病小鼠体内的GSH、SOD、过氧化氢酶、GPx等抗氧化酶控制到正常水平，证明了其在高血糖时的抗氧化作用。在糖尿病小鼠中，AuNPs对血糖水平、血脂和血清生化特征的持续控制接近于对照小鼠，这表明它们在控制和增加器官功能以更好地利用血糖方面发挥了有效作用。组织病理学和血液学研究表明，当剂量为2.5毫克/公斤体重/天时，金纳米颗粒对重要器官具有无毒和保护作用。ICP-MS分析揭示了金纳米颗粒在重要脏器中的生物分布，显示脾脏中AuNPs的积累相对大于其他脏器。结果揭示了AuNPs作为抗氧化剂的有效作用，通过抑制ROS的形成，清除自由基；从而增加抗氧化防御酶，创造对高血糖状况的持续控制，从而激发aunp作为糖尿病治疗及其并发症的经济治疗药物的潜力。
Oxidative stress is imperative for its morbidity towards diabetic complications, where abnormal metabolic milieu as a result of hyperglycemia, leads to the onset of several complications. A biological antioxidant capable of inhibiting oxidative stress mediated diabetic progressions; during hyperglycemia is still the need of the era. The current study was performed to study the effect of biologically synthesized gold nanoparticles (AuNPs) to control the hyperglycemic conditions in streptozotocin induced diabetic mice. The profound control of AuNPs over the anti oxidant enzymes such as GSH, SOD, Catalase and GPx in diabetic mice to normal, by inhibition of lipid peroxidation and ROS generation during hyperglycemia evidence their anti-oxidant effect during hyperglycemia. The AuNPs exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic mice near to the control mice provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological and hematological studies revealed the non-toxic and protective effect of the gold nanoparticles over the vital organs when administered at dosage of 2.5 mg/kilogram.body.weight/day. ICP-MS analysis revealed the biodistribution of gold nanoparticles in the vital organs showing accumulation of AuNPs in the spleen comparatively greater than other organs. The results obtained disclose the effectual role of AuNPs as an anti-oxidative agent, by inhibiting the formation of ROS, scavenging free radicals; thus increasing the anti-oxidant defense enzymes and creating a sustained control over hyperglycemic conditions which consequently evoke the potential of AuNPs as an economic therapeutic remedy in diabetic treatments and its complications.