Online coupling of capillary electrophoresis with direct analysis in real time mass spectrometry.
Online coupling of capillary electrophoresis with direct analysis in real time mass spectrometry.
复制标题
DOI：
10.1021/ac303450v
复制
发表时间：
2013-01
影响因子：
7.4
通讯作者：
Cuilan Chang;Gege Xu;Yu Bai;Chengsen Zhang;Xianjiang Li;Min Li;Yi Liu;Huwei Liu
中科院分区：
文献类型：
--
作者：
Cuilan Chang;Gege Xu;Yu Bai;Chengsen Zhang;Xianjiang Li;Min Li;Yi Liu;Huwei Liu
通过同轴针尖接口实现了毛细管电泳质谱(DART-MS)与环境直接分析的在线联用。毛细管电泳柱(CE)洗脱出的分析物被DART产生的亚稳氦通量直接电离后转移到MS中进行检测，实现了在线分离和同时检测。与传统的CE-ESI-MS相比，CE-DART-MS可以承受更高浓度的洗涤剂和盐类，避免了收集CE流出物和清洗界面的困难，简化了实验程序，缩短了分析时间。毛细管区带电泳法(CZE)和胶束电动毛细管色谱(MEKC)对4-氨基安替比林、佐米曲坦和奎宁的混合物进行了验证。该方法重现性好，相对标准偏差(RSD；n=5)为0.56~1.23%，峰面积为2.01~7.41%。4-氨基安替比林在0.9995.0 1~0.5 0 mg/m L范围内可进行定量分析，线性相关系数为0.2 5%，检出限为14.7fm ol。与CE-ESI-MS相比，非挥发性盐和洗涤剂对离子的抑制作用被有效地降低。当浓度达到100 mM的硼酸钠和30 mM的十二烷基硫酸钠(在30 mM的硼酸钠缓冲液中)时，信号强度保持不变。此外，该方法还成功地应用于白茶中咖啡因的检测。
The online coupling of capillary electrophoresis with ambient direct analysis in real time mass spectrometry (DART-MS) was realized by a coaxial tip interface. The analytes eluted from capillary electrophoresis (CE) were directly ionized by the metastable helium flux produced by DART and transferred into MS for the detection, with which the online separation and simultaneous detection were achieved. The CE-DART-MS can tolerate higher concentrations of detergents and salts than traditional CE-electrospray ionization (ESI)-MS and avoided the difficulties of collecting CE effluent and cleaning the interface, which simplified the experimental procedures and shortened the analysis time. The performance of the technique was successfully verified by capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) using a mixture of 4-aminoantipyrine, zolmitriptan, and quinine. This online technique showed good repeatability with the relative standard deviations (RSDs; n = 5) of 0.56-1.23% for the retention times and 2.01-7.41% for the peak areas. The quantitative analysis of 4-aminoantipyrine was accomplished in the range of 0.01-0.50 mg/mL with the linear correlation coefficient of 0.9995 and limit of detection of 14.7 fmol. Compared with CE-ESI-MS, the ion suppression effects of nonvolatile salts and detergents were efficiently minimized. The signal intensity remained constant when the concentrations reached 100 mM for sodium borate and 30 mM for SDS (in 30 mM sodium borate buffer). In addition, the proposed method was successfully applied to the detection of the endogenous caffeine in Chinese white tea.