Phenotypic screening for heart rate variability in the mouse
Phenotypic screening for heart rate variability in the mouse
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DOI：
10.1152/ajpheart.2000.279.2.h733
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发表时间：
2000-08-01
影响因子：
4.8
通讯作者：
Berul, CI
中科院分区：
文献类型：
--
作者：
Gehrmann, J;Hammer, PE;Berul, CI
我们开发了一种用于小鼠心率（HR）变异性（HRV）分析的技术，用于表征由迷走神经和交感神经活动调制的HR动力学。小鼠是研究生物过程的主要动物模型。目前已有携带基因突变的小鼠品系，为深入了解心电疾病的分子机制提供了基础。未来的进展取决于增强对这些基本机制的理解，以及对小鼠心血管生理功能分析方法的实施。通过遥测技术，标准的时域和频域测量心率变异性计算和自主神经阻滞，压力反射敏感性测试进行。高频分量中的HR调制主要由副交感神经系统介导，而低频分量受副交感神经系统和交感神经系统两者的影响。所提出的技术和协议允许评估的自主调节的小鼠HR。表型筛选小鼠的HR调节将进一步提高小鼠作为一个模型的遗传性电生理人类疾病的价值。
We developed a technology for heart rate (HR) variability (HRV) analysis in the mouse for characterization of HR dynamics, modulated by vagal and sympathetic activity. The mouse is the principal animal model for studying biological processes. Mouse strains are now available harboring gene mutations providing fundamental insights into molecular mechanisms underlying cardiac electrical diseases. Future progress depends on enhanced understanding of these fundamental mechanisms and the implementation of methods for the functional analysis of mouse cardiovascular physiology. By telemetric techniques, standard time and frequency-domain measures of HRV were computed with and without autonomic blockade, and baroreflex sensitivity testing was performed. HR modulation in the high-frequency component is predominantly mediated by the parasympathetic nervous system, whereas the low-frequency component is under the influence of both the parasympathetic and sympathetic systems. The presented technology and protocol allow for assessment of autonomic regulation of the murine HR. Phenotypic screening for HR regulation in mice will further enhance the value of the mouse as a model of heritable electrophysiological human disease.