Genomics reveals a genetically isolated population of the Pacific white‐sided dolphin ( <i>Lagenorhynchus obliquidens</i> ) distributed in the Sea of Japan
Genomics reveals a genetically isolated population of the Pacific white‐sided dolphin ( <i>Lagenorhynchus obliquidens</i> ) distributed in the Sea of Japan
复制标题
基因组学揭示了分布在日本海的太平洋白海豚（<i>Lagenorhynchus obliquidens</i>）的基因分离种群
DOI：
10.1111/mec.16797
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Kato Haruhiko
中科院分区：
文献类型：
--
作者：
Suzuki Miwa;Ohno Kaho;Sawayama Eitaro;Morinaga Shin‐Ichi;Kishida Takushi;Matsumoto Teruyo;Kato Haruhiko
动物在开放环境（如海洋）中遗传隔离的过程尚未完全阐明。在日本海同域地观察到太平洋白色侧棘鲷的形态学不同种群。然而，仅使用有限的线粒体位点或微卫星DNA的遗传研究未能证明存在遗传上不同的种群。在这里，为了揭示种群结构，我们使用2018-2021年从水族馆的所有国内圈养个体中收集的单核苷酸多态性（SNP）分析了全基因组种群遗传数据，其中大部分来自野外，以及一些搁浅的个体，共同覆盖了日本周围的沿海水域（n= 123）。进行多重简单序列间重复序列基因分型-测序分析以获得SNP数据。主坐标分析和聚类分析表明，两个遗传上不同的群体存在，很少有群体间的基因流揭示。此外，基因型分离反映在外部形态型的差异。此外，基于来自每个种群的个体的全基因组序列的种群人口统计学分析表明，末次冰期期间的海平面变化可能导致该物种在日本海的有限区域内的异域分化，该群体随后与其他种群共享分布区域。这些发现有助于了解海洋中遗传隔离的同域种群的形成。
The processes by which animals become genetically isolated in an open environment such as the ocean have not yet been fully elucidated. Morphologically different populations of Pacific white‐sided dolphinLagenorhynchus obliquidensare observed sympatrically in the Sea of Japan. However, genetic studies that exclusively used limited mitochondrial loci or microsatellite DNA have failed to demonstrate the existence of genetically distinct populations. Here, to reveal the population structure, we analysed genome‐wide population genetic data using single nucleotide polymorphisms (SNPs) gathered in 2018–2021 from all domestic captive individuals in aquaria, the majority of which originated from the wild, as well as from some stranded individuals, together covering a wide area of coastal water around Japan (n= 123). Multiplexed intersimple sequence repeat genotyping‐by‐sequencing analysis was performed to obtain the SNP data. Principal coordinate analysis and the clustering methodstructureindicated that two genetically‐distinct populations exist, with little interpopulation gene flow revealed. In addition, the genotypic segregation was reflected in differences in external morphotype. Furthermore, a population demographic analysis based on the whole‐genome sequences of an individual from each population indicated that sea‐level changes during the Last Glacial Period probably led to allopatric divergence of this species in a limited area of the Sea of Japan, with that group subsequently sharing a distribution area with the other population. These findings yield insights into the formation of genetically isolated sympatric populations in the ocean.