Plant allelochemicals affect tolerance of polyphagous lepidopteran pest Helicoverpa armigera (Hubner) against insecticides
Plant allelochemicals affect tolerance of polyphagous lepidopteran pest Helicoverpa armigera (Hubner) against insecticides
复制标题
植物化感物质影响多食性鳞翅目害虫棉铃虫 (Hübner) 对杀虫剂的耐受性
DOI：
10.1016/j.pestbp.2018.12.009
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发表时间：
2019-02-01
影响因子：
4.7
通讯作者：
Song, Yuan-Yuan
中科院分区：
文献类型：
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作者：
Chen, Shi;Elzaki, Mohammed Esmail Abdalla;Song, Yuan-Yuan
棉铃虫Helicoverpa armigera（Hubner）（鳞翅目：夜蛾科）是鳞翅目昆虫的一种多食性害虫，在世界范围内广泛分布，寄主范围广。但H.棉铃虫对各种杀虫剂和其寄主植物中防御性化感物质的适应性尚不完全清楚。因此，本研究探讨了植物化感物质的摄入对棉铃虫幼虫对灭多威和毒死蜱杀虫剂耐受性的影响。armigera及其可能的机制。筛选了12种植物化感物质，研究其对幼虫灭多威敏感性的影响。其中黄酮、香豆素、丁布（2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮）和visnagin显著降低了幼虫对灭多威的敏感性。细胞色素P450抑制剂胡椒基丁醚（PBO）的应用显着增加灭多威处理的幼虫的死亡率。与此相反，PBO除了显着降低毒死蜱处理的幼虫的死亡率。此外，化感物质的消耗增强谷胱甘肽S-转移酶，羧酸酯酶，细胞色素P450和乙酰胆碱酯酶在中肠和脂肪体的活动。qRT-PCR分析证实了中肠和脂肪体中的P450基因、CYP 6 B2、CYP 6 B6和CYP 6 B7被4种化感物质诱导。最后，通才H.棉铃虫可以利用寄主植物的化感物质来增强对杀虫剂的防御能力。
Cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), is a polyphagous lepidopteran pest distributed worldwide with a broad spectrum of host plants. However, the mechanism of H. armigera adaptation to various insecticides and defensive allelochemicals in its host plants is not fully understood. Therefore, this study examined the influence of consumption of plant allelochemicals on larval tolerance to methomyl and chlorpyrifos insecticides in H. armigera and its possible mechanism. Twelve plant allelochemicals were screened to evaluate their effects on larval sensitivity to methomyl. Of which flavone, coumarin, DIMBOA (2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one) and visnagin significantly reduced larval sensitivity to methomyl. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly increased the mortality of methomyl-treated larvae. In contrast, PBO addition significantly decreased the mortality of chlorpyrifos-treated larvae. Moreover, allelochemical consumption enhanced the activities of glutathione S-transferase, carboxylesterase, cytochrome P450 and acetylcholinesterase in the midgut and fat body. The qRT-PCR analysis confirms that P450 genes, CYP6B2, CYP6B6 and CYP6B7 were induced by the four allelochemicals in the midguts and the fat bodies. In conclusion, the generalist H. armigera can take benefit of plant allelochemicals from its host plants to elaborate its defense against insecticides.