Expression of nuclear receptor-transcription factor genes during Aedes aegypti midgut metamorphosis and the effect of methoprene on expression
Expression of nuclear receptor-transcription factor genes during Aedes aegypti midgut metamorphosis and the effect of methoprene on expression
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DOI：
10.1016/j.ibmb.2005.01.019
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发表时间：
2005-06-01
影响因子：
3.8
通讯作者：
Murray, J
中科院分区：
文献类型：
--
作者：
Nishiura, JT;Ray, K;Murray, J
将4龄蚊子暴露于保幼激素类似物甲氧普烯，通过干扰变态来阻止成虫的出现。一个被破坏的变质过程是中肠重塑。为了研究这种情况发生的分子机制，埃及伊蚊（L.）采用真实的实时荧光定量PCR方法对4龄幼虫进行了检测。结果表明，在未处理的幼虫中，转录因子AHR 3和AaE 75 B的表达在末次幼虫蜕皮后24 h内增加，AaEcR-B、AaUSP-a和AaBFTZ-F1的转录在约24 h后增加，而AaE 75 A的转录在幼虫蜕皮前增加。AaUSP-b基因在4龄幼虫中表达一致。研究了甲氧普烯暴露对中肠重塑过程中转录因子基因表达的影响。结果表明，在剂量和阶段依赖性的方式，甲氧普烯影响增加的表达，通常发生在中肠重塑。甲氧普烯对变形中肠重塑和转录因子基因表达的同步作用表明这两个过程是相关的。(c)2005爱思唯尔有限公司保留所有权利。
Exposure of mosquito 4th instars to the juvenile hormone analogue methoprene prevents the emergence of adults by interfering with metamorphosis. One metamorphic processes that is disrupted is midgut remodeling. To investigate the molecular mechanisms by which this occurs, the pattern of transcription factor gene expression during the Aedes aegypti (L.) 4th instar was investigated by the method of real time PCR. The results indicate that in untreated larvae, expression of transcription factors genes AHR3 and AaE75B increases within 24h after the last larval-larval molt, transcription of AaEcR-B, AaUSP-a and AaBFTZ-F1 increases approximately 24 h later, and transcription of AaE75A increases just before the larval-pupal molt. There is uniform expression of AaUSP-b throughout the 4th instar. The effect of methoprene exposure on transcription factor gene expression during midgut remodeling was investigated. The results indicate that, in a dose and stage dependent manner, methoprene affects increases in expression that normally occur during midgut remodeling. The coincident effects of methoprene on metamorphic midgut remodeling and on transcription factor gene expression suggests that the two processes are related. (c) 2005 Elsevier Ltd. All rights reserved.