Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP
Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP
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DOI：
10.1016/s1567-133x(01)00011-4
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发表时间：
2001-08-01
影响因子：
1.2
通讯作者：
Kitamoto, Toshihiro
中科院分区：
文献类型：
--
作者：
Salvaterra, Paul M.;Kitamoto, Toshihiro
利用果蝇胆碱能基因5‘侧翼7.4kb的DNA来驱动Gal4的表达，我们基本上可以看到与UAS-GFP(S65T)报告基因遗传重组后的所有胆碱能神经元和神经堆。与以前的方法不同的是，在同一样本中可以观察到体细胞和神经丛。荧光强度足够强，可以在所有发育阶段的活体动物中观察到。从整装制剂的共焦切片进行的三维重建揭示了神经系统不同区域之间广泛的胆碱能联系。
Using 7.4 kb of 5' flanking DNA from the Drosophila cholinergic gene locus to drive Gal4 expression we can visualize essentially all cholinergic neurons and neuropiles after genetic recombination with a UAS-GFP (S65T) reporter gene. In contrast to previous methods somata and neuropiles can be observed in the same samples. Fluorescence intensity is strong enough to allow observations in live animals at all developmental stages. Three-dimensional reconstructions made from confocal sections of whole-mount preparations reveal the extensive cholinergic connections among various regions of the nervous system.