KINETIC STUDY OF RABBIT MUSCLE PYRUVATE-KINASE
KINETIC STUDY OF RABBIT MUSCLE PYRUVATE-KINASE
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DOI：
10.1042/bj1310223
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发表时间：
1973-01-01
影响因子：
4.1
通讯作者：
MACFARLANE, N
中科院分区：
文献类型：
--
作者：
AINSWORTH, S;MACFARLANE, N
本文报道了兔肌丙酮酸激酶催化磷酸烯醇式丙酮酸、二磷酸腺苷和镁离子反应的动力学。实验结果表明，该反应机理为平衡无序型，底物和产物为磷酸烯醇式丙酮酸、ADP、镁离子、丙酮酸和镁三磷酸腺苷，丙酮酸、ADP和镁离子之间的末端络合物是无规形成的，与自身和其他底物络合物平衡存在。测定了反应的米氏常数、离解常数和抑制常数，并与以前工作人员确定的值进行了比较。
The paper reports a study of the kinetics of the reaction between phosphoenolpyruvate, ADP and Mg2+catalysed by rabbit muscle pyruvate kinase. The experimental results indicate that the reaction mechanism is equilibrium random-order in type, that the substrates and products are phosphoenolpyruvate, ADP, Mg2+, pyruvate and MgATP, and that dead-end complexes, between pyruvate, ADP and Mg2+, form randomly and exist in equilibrium with themselves and other substrate complexes. Values were determined for the Michaelis, dissociation and inhibition constants of the reaction and are compared with values ascertained by previous workers.