Kinetic parameters of 3-[123I]iodo-L-α-methyl tyrosine ([123I]IMT) transport in human GOS3 glioma cells
Kinetic parameters of 3-[123I]iodo-L-α-methyl tyrosine ([123I]IMT) transport in human GOS3 glioma cells
复制标题
DOI：
10.1016/s0969-8051(01)00191-3
复制
发表时间：
2001-04-01
影响因子：
3.1
通讯作者：
Schober, O
中科院分区：
文献类型：
--
作者：
Riemann, B;Kopka, K;Schober, O
放射性标记氨基酸3-[I-123]碘-L-α-甲基酪氨酸([I-123]TMT)是一种很有前途的单光子发射断层扫描(SPECT)诊断和监测脑肿瘤的工具。然而，关于[I-123]IMT在人脑胶质瘤细胞中摄取的精确动力学还知之甚少。对[I-123]IMT在人GOS3胶质瘤细胞中转运的动力学分析得到了高亲和力的表观米氏常数(K-m=20.1+/-1.5um)。最大转运速度(V-max)为34.8+/-1.9nmol/mg蛋白/10min。竞争性抑制实验表明[I-123]IMT转运主要由钠非依赖性系统L(C)2001 Elsevier Science Inc.介导。版权所有。
The radiolabelled amino acid 3-[I-123]iodo-L-alpha -methyl tyrosine ([I-123]TMT) is a promising tool for the diagnosis and monitoring of brain tumors using single-photon emission tomography (SPECT). However, little is known about the precise kinetics of [I-123]IMT uptake in human glioma cells. The kinetic analysis of [I-123]IMT transport in human GOS3 glioma cells yielded a high-affinity apparent Michaelis constant (K-m = 20.1 +/- 1.5 muM). The maximum transport velocity (V-max) amounted to 34.8 +/- 1.9 nmol/mg protein/10 min. Competitive inhibition experiments revealed that [I-123]IMT transport is mediated principally by the sodium-independent system L. (C) 2001 Elsevier Science Inc. All rights reserved.