MULTIPLE RETINOID-RESPONSIVE RECEPTORS IN A SINGLE CELL - FAMILIES OF RETINOID X RECEPTORS AND RETINOIC ACID RECEPTORS IN THE XENOPUS EGG
MULTIPLE RETINOID-RESPONSIVE RECEPTORS IN A SINGLE CELL - FAMILIES OF RETINOID X RECEPTORS AND RETINOIC ACID RECEPTORS IN THE XENOPUS EGG
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DOI：
10.1073/pnas.89.6.2321
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发表时间：
1992-03-15
影响因子：
11.1
通讯作者：
DEROBERTIS, EM
中科院分区：
文献类型：
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作者：
BLUMBERG, B;MANGELSDORF, DJ;DEROBERTIS, EM
在寻找早期发育中表达的核激素受体时，我们发现非洲爪蟾卵含有来自两个视黄酸受体基因（xRAR-α和xRAR-gamma）和两个视黄酸“X”受体基因（xRXR-α和xRXR-gamma）的mRNA。我们还表明，RXR 是至少三个基因家族的成员，从而将编码视黄酸响应转录因子的基因数量扩大到六个。除 xRXR-gamma 外，这些母体 mRNA 在原肠胚形成前被降解。分离的 RXR 被视黄酸和 3,4-二脱氢视黄酸不同程度地激活。综合考虑，这四种受体为视黄酸对早期发育的多效性作用提供了分子基础，并且它们的表达模式表明视黄酸在胚胎决定的最早阶段的作用。
In a search for nuclear hormone receptors expressed in early development we found that Xenopus laevis eggs contain mRNAs from two retinoic acid receptor genes (xRAR-alpha and xRAR-gamma) and two retinoid "X" receptor genes (xRXR-alpha and xRXR-gamma). We also show that RXRs are members of a family of at least three genes, thus expanding the number of genes encoding retinoic acid-responsive transcription factors to six. With the exception of xRXR-gamma, these maternal mRNAs are degraded before gastrulation. The RXRs isolated are differentially activated by retinoic acid and by 3,4-didehydroretinoic acid. Considered together, these four receptors provide a molecular basis for the pleiotropic effects of retinoic acid on early development, and their pattern of expression suggests a role for retinoic acid at the earliest stages of embryonic determination.