In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kützing and Spirulina plantensis.
In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kützing and Spirulina plantensis.
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食用蓝绿藻（Nostoc commune var.）的体外和体内安全性评估。
DOI：
10.1016/j.fct.2011.03.052
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Lee,Jiyoung
中科院分区：
文献类型：
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作者：
Yang,Yue;Park,Youngki;Cassada,DavidA;Snow,DanielD;Rogers,DouglasG;Lee,Jiyoung
几个世纪以来，蓝绿藻（BGA）一直被用作食物和草药。然而，其消费的安全性尚未得到很好的研究。本文研究了普通念珠藻（Nostoc communevar.sphaeroides Kützing，NO）和钝顶螺旋藻（Spirulina platensis，SP）的体外和体内毒性。当HepG 2细胞与0-500 μg/ml的己烷、氯仿、甲醇和NO和SP的水可提取级分一起孵育时，细胞存活率保持在70-80%左右。2.5%或5%的NO和SP（wt/wt）持续6个月。对于两种性别，富含BGA的饮食并没有引起体重增加和血浆丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶浓度的显着异常，除了在6个月的雄性小鼠中补充2.5%NO后血浆ALT水平显着增加。然而，肝脏的组织学分析表明，BGA与对照组相比没有造成显着的肝损伤。总之，我们的研究结果表明，NO和SP是免费的MC和长期的饮食补充高达5%的BGA可以消耗没有明显的毒副作用。
Blue-green algae (BGA) have been consumed as food and herbal medicine for centuries. However, safety for their consumption has not been well investigated. This study was undertaken to evaluate in vitro and in vivo toxicity of cultivatedNostoc communevar.sphaeroides Kützing(NO) andSpirulina platensis(SP). Neither NO nor SP contained detectable levels of microcystin (MC)-LA, MC-RR, MC-LW and MC-LR by LC/MS/MS. Cell viability remained ∼70–80% when HepG2 cells were incubated with 0–500 μg/ml of hexane, chloroform, methanol and water-extractable fractions of NO and SP. Four-week-old male and female C57BL/6J mice were fed an AIN-93G/M diet supplemented with 0%, 2.5% or 5% of NO and SP (wt/wt) for 6 months. For both genders, BGA-rich diets did not induce noticeable abnormality in weight gain and plasma alanine aminotransferase (ALT) and aspartate aminotransferase concentrations except a significant increase in plasma ALT levels by 2.5% NO supplementation in male mice at 6 month. Histopathological analysis of livers, however, indicated that BGA did not cause significant liver damage compared with controls. In conclusion, our results suggest that NO and SP are free of MC and the long-term dietary supplementation of up to 5% of the BGA may be consumed without evident toxic side-effects.