NOVEL REGULATORS OF BONE-FORMATION - MOLECULAR CLONES AND ACTIVITIES
NOVEL REGULATORS OF BONE-FORMATION - MOLECULAR CLONES AND ACTIVITIES
复制标题
DOI：
10.1126/science.3201241
复制
发表时间：
1988-12-16
期刊：
影响因子：
56.9
通讯作者：
WANG, EA
中科院分区：
文献类型：
--
作者：
WOZNEY, JM;ROSEN, V;WANG, EA
来源于骨的蛋白质提取物可以启动以软骨形成开始并以从头骨形成结束的过程。这种提取物的关键成分，称为骨形态发生蛋白（BMP），指导软骨和骨形成以及在此过程中由动物提供的组成成分长期以来一直不清楚。氨基酸序列已从牛骨的高度纯化的BMP制剂中获得。现在，已经分离出与该BMP制剂中存在的三种多肽相对应的人互补DNA克隆，并且已经获得了重组人蛋白的表达。这三种蛋白（BMP-1、BMP-2A和BMP-3）中的每一种似乎都能够独立地诱导体内软骨的形成。两种编码的蛋白质（BMP-2A和BMP-3）是TGF-β的新成员。BMP-1是一个新的调控分子。
Protein extracts derived from bone can initiate the process that begins with cartilage formation and end in de novo bone formation. The critical components of this extract, termed bone morphogenetic protein (BMP), that direct cartilage and bone formation as well as the constitutive elements supplied by the animal during this process have long remained unclear. Amino acid sequence has been derived from a highly purified preparation of BMP from bovine bone. Now, human complementary DNA clones corresponding to three polypeptides present in this BMP preparation have been isolated, and expression of the recombinant human proteins have been obtained. Each of the three (BMP-1, BMP-2A, and BMP-3) appears to be independently capable of inducing the formation of cartilage in vivo. Two of the encoded proteins (BMP-2A and BMP-3) are new members of the TGF-.beta. supergene family, while the third, BMP-1, appears to be a novel regulatory molecule.