Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin.
Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin.
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DOI：
10.1016/j.celrep.2023.113254
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发表时间：
2023-10-31
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin. Santos et al. demonstrate that ebolaviruses spread to neighboring cells through intercellular connections. They develop methodologies to quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. The antibody BDBV223 has the property of inducing virus accumulation at the plasma membrane, dependent on the expression of BST2.
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影响因子：
32.4
作者：
Gilchuk P;Kuzmina N;Ilinykh PA;Huang K;Gunn BM;Bryan A;Davidson E;Doranz BJ;Turner HL;Fusco ML;Bramble MS;Hoff NA;Binshtein E;Kose N;Flyak AI;Flinko R;Orlandi C;Carnahan R;Parrish EH;Sevy AM;Bombardi RG;Singh PK;Mukadi P;Muyembe-Tamfum JJ;Ohi MD;Saphire EO;Lewis GK;Alter G;Ward AB;Rimoin AW;Bukreyev A;Crowe JE Jr
通讯作者：
Crowe JE Jr
影响因子：
64.5
作者：
Flyak AI;Shen X;Murin CD;Turner HL;David JA;Fusco ML;Lampley R;Kose N;Ilinykh PA;Kuzmina N;Branchizio A;King H;Brown L;Bryan C;Davidson E;Doranz BJ;Slaughter JC;Sapparapu G;Klages C;Ksiazek TG;Saphire EO;Ward AB;Bukreyev A;Crowe JE Jr
通讯作者：
Crowe JE Jr
影响因子：
64.8
作者：
Cote, Marceline;Misasi, John;Ren, Tao;Bruchez, Anna;Lee, Kyungae;Filone, Claire Marie;Hensley, Lisa;Li, Qi;Ory, Daniel;Chandran, Kartik;Cunningham, James
通讯作者：
Cunningham, James
影响因子：
6.7
作者：
Ilinykh PA;Santos RI;Gunn BM;Kuzmina NA;Shen X;Huang K;Gilchuk P;Flyak AI;Younan P;Alter G;Crowe JE Jr;Bukreyev A
通讯作者：
Bukreyev A
DOI：
10.1126/science.aaa8651
发表时间：
2015-07-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fibriansah G;Ibarra KD;Ng TS;Smith SA;Tan JL;Lim XN;Ooi JS;Kostyuchenko VA;Wang J;de Silva AM;Harris E;Crowe JE Jr;Lok SM
通讯作者：
Lok SM