Isoorientin induces apoptosis through mitochondrial dysfunction and inhibition of PI3K/Akt signaling pathway in HepG2 cancer cells
Isoorientin induces apoptosis through mitochondrial dysfunction and inhibition of PI3K/Akt signaling pathway in HepG2 cancer cells
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异荭草素通过线粒体功能障碍和抑制 PI3K/Akt 信号通路诱导 HepG2 癌细胞凋亡
DOI：
10.1016/j.taap.2012.09.022
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发表时间：
2012-11-15
影响因子：
3.8
通讯作者：
Liu, Xuebo
中科院分区：
文献类型：
--
作者：
Yuan, Li;Wang, Jing;Liu, Xuebo
异荭草苷（Isoorientin， ISO）是一种黄酮类化合物，可从毛竹（Phyllostachys pubescens）、黄花蒿（Patrinia）和lusitanicum等植物中提取；然而，其生物活性仍然知之甚少。本研究探讨了ISO对人肝母细胞瘤（HepG2）细胞凋亡的影响及其可能的机制。结果表明，ISO在HepG2细胞中呈剂量依赖性诱导细胞死亡，但对人肝细胞（HL-7702）和水鼠肝细胞（BRL-3A）在指定浓度下无毒性。iso诱导的细胞死亡包括凋亡，其特征是细胞核收缩、聚腺苷核糖聚合酶（PARP）裂解和DNA断裂。ISO显著(p)
Isoorientin (ISO) is a flavonoid compound that can be extracted from several plant species, such as Phyllostachys pubescens, Patrinia, and Drosophyllum lusitanicum; however, its biological activity remains poorly understood. The present study investigated the effects and putative mechanism of apoptosis induced by ISO in human hepatoblastoma cancer (HepG2) cells. The results showed that ISO induced cell death in a dose-dependent manner in HepG2 cells, but no toxicity in human liver cells (HL-7702) and buffalo rat liver cells (BRL-3A) treated with ISO at the indicated concentrations. ISO-induced cell death included apoptosis which characterized by the appearance of nuclear shrinkage, the cleavage of poly (ADP-ribose) polymerase (PARP) and DNA fragmentation. ISO significantly (p