Relationship between enamel fluorosis severity and fluoride content.
Relationship between enamel fluorosis severity and fluoride content.
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DOI：
10.1016/j.jdent.2016.01.007
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发表时间：
2016-03
影响因子：
4.4
通讯作者：
Soto-Rojas AE
中科院分区：
文献类型：
--
作者：
Martinez-Mier EA;Shone DB;Buckley CM;Ando M;Lippert F;Soto-Rojas AE
釉质氟中毒是牙齿发育过程中长期暴露在高氟环境中引起的一种矿化程度较低的疾病。以往关于牙釉质氟化物含量和氟斑牙严重程度之间关系的研究一直不明确。目前的研究目的是比较目测和组织学评估的氟中毒严重程度与釉质氟含量。使用Thylstrup和FeJerskov指数对112颗拔除的牙齿进行氟中毒的目测检查。记录每颗牙的萌出情况。将牙齿切成100μm切片，在偏光显微镜下观察组织学变化。牙齿被分类为健康、轻度、中度或重度氟中毒，从视觉和组织学上看。在距外表面30、60和90μm处将其切割成2×2 mm的正方形，进行氟含量测定(显微活检)。重度氟中毒萌出牙在30、60和90μm处的平均氟含量显著高于健牙。轻、中、重度氟中毒未萌牙在30μm的平均氟含量显著高于健康牙，轻、重度氟中毒的未萌牙在60μm的平均氟含量显著高于健康牙，而只有重度氟中毒的未萌牙在90μm的平均氟含量显著高于健康牙，重度氟斑牙和重度氟斑牙的牙釉质氟含量均高于健康牙。有关牙釉质中氟含量的数据将进一步加深我们对其生物学特性的了解，从而在硬组织疾病和条件的管理中发挥作用。
Enamel fluorosis is a hypomineralization caused by chronic exposure to high levels of fluoride during tooth development. Previous research on the relationship between enamel fluoride content and fluorosis severity has been equivocal. The current study aimed at comparing visually and histologically assessed fluorosis severity with enamel fluoride content. Extracted teeth (n=112) were visually examined using the Thylstrup and Fejerskov Index for fluorosis. Eruption status of each tooth was noted. Teeth were cut into 100 μm slices to assess histological changes with polarized light microscopy. Teeth were categorized as sound, mild, moderate, or severe fluorosis, visually and histologically. They were cut into squares (2×2 mm) for the determination of fluoride content (microbiopsy) at depths of 30, 60 and 90 μm from the external surface. Erupted teeth with severe fluorosis had significantly greater mean fluoride content at 30, 60 and 90 μm than sound teeth. Unerupted teeth with mild, moderate and severe fluorosis had significantly greater mean fluoride content than sound teeth at 30 μm; unerupted teeth with mild and severe fluorosis had significantly greater mean fluoride content than sound teeth at 60 μm, while only unerupted teeth severe fluorosis had significantly greater mean fluoride content than sound teeth at 90 μm. Both erupted and unerupted severely fluorosed teeth presented higher mean enamel fluoride content than sound teeth. Data on fluoride content in enamel will further our understanding of its biological characteristics which play a role in the management of hard tissue diseases and conditions.