Subunit-dependent postsynaptic expression of kainate receptors on hippocampal interneurons in area CA1.
Subunit-dependent postsynaptic expression of kainate receptors on hippocampal interneurons in area CA1.
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DOI：
10.1523/jneurosci.4788-08.2009
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发表时间：
2009-01-14
期刊：
影响因子：
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通讯作者：
Frerking M
中科院分区：
文献类型：
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作者：
Wondolowski J;Frerking M
红藻氨酸受体（KAR）仅在选定的神经元子集中促进突触后兴奋。为了定义指定KARs突触后表达的参数，我们研究了KARs对CA 1区海马中间神经元EPSC的贡献。放射层/腔隙分子层（SR/SLM）中的中间神经元表达含和不含GluR 5亚基的KAR，但KAR介导的EPSC主要（如果不是全部）由含GluR 5的KAR产生。突触外谷氨酸溢出深刻地招募AMPAR，对KAR影响不大，表明KAR比AMPAR更精确地靶向突触。然而，具有常规AMPAR组分的自发EPSC没有可分辨的KAR贡献，这表明对诱发EPSC有贡献的KAR位于一组不同的突触。含有GluR 5的KARs在东方层的中间神经元上对EPSC没有实质性的贡献。我们的结论是，KARs是本地化的细胞类型，突触，和亚单位选择性机制的突触。
Kainate receptors (KARs) contribute to postsynaptic excitation in only a select subset of neurons. To define the parameters that specify the postsynaptic expression of KARs, we examined the contribution of KARs to EPSCs on hippocampal interneurons in area CA1. Interneurons in stratum radiatum/lacunosum-moleculare (SR/SLM) express KARs both with and without the GluR5 subunit, but KAR-mediated EPSCs are generated mainly, if not entirely, by GluR5-containing KARs. Extrasynaptic glutamate spillover profoundly recruits AMPARs with little effect on KARs, indicating that KARs are targeted at the synapse more precisely than AMPARs. However, spontaneous EPSCs with a conventional AMPAR component did not have a resolvable contribution of KARs, suggesting that the KARs that contribute to the evoked EPSCs are at a distinct set of synapses. GluR5-containing KARs on interneurons in stratum oriens do not contribute substantially to the EPSC. We conclude that KARs are localized to synapses by cell type-, synapse-, and subunit-selective mechanisms.