Protection of MN-rgp120-immunized chimpanzees from heterologous infection with a primary isolate of human immunodeficiency virus type 1
Protection of MN-rgp120-immunized chimpanzees from heterologous infection with a primary isolate of human immunodeficiency virus type 1
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DOI：
10.1093/infdis/173.1.52
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发表时间：
1996-01-01
影响因子：
6.4
通讯作者：
Obijeski, JF
中科院分区：
文献类型：
--
作者：
Berman, PW;Murthy, KK;Obijeski, JF
用HIV-1 MN株重组gp 120免疫的3只黑猩猩和1只对照动物静脉注射HIV-1（SF 2）的原代分离株进行攻击。在感染后2周开始，通过病毒培养、聚合酶链反应和多种血清学测定在对照动物中检测到病毒感染，在12个月的随访期间，在任何gp 120免疫的动物中均未检测到HIV-1感染的标志物，来自gp 120免疫的黑猩猩的抗血清不能中和外周血单核细胞（PBMC）中培养的攻击病毒，这些研究表明，免疫与重组gp 120来源于T细胞适应的分离预防感染的异源的主要分离的HIV-1。结果表明，在体外病毒中和试验利用原代分离培养的PBMC可能是不完善的指标，在体内的保护。
Three chimpanzees immunized with recombinant gp120 from human immunodeficiency virus type 1 (HIV-1) strain MN and 1 control animal were challenged intravenously with a primary isolate of HIV-1(SF2). Viral infection was detected in the control animal by viral culture, polymerase chain reaction, and multiple serologic assays beginning 2 weeks after infection, Markers of HIV-1 infection were not detected in any of the gp120-vaccinated animals during 12 months of follow-up, Antisera from the gp120-immunized chimpanzees were unable to neutralize the challenge virus cultured in peripheral blood mononuclear cells (PBMC), These studies demonstrate that immunization with recombinant gp120 derived from a T cell-adapted isolate prevented infection by a heterologous primary isolate of HIV-1. The results suggest that in vitro virus neutralization assays utilizing primary isolates cultured in PBMC may be imperfect indicators of protection in vivo.