THE EFFECT OF DEXAMETHASONE ADMINISTRATION ON RAT INTESTINAL PERMEABILITY - THE ROLE OF BACTERIAL ADHERENCE
THE EFFECT OF DEXAMETHASONE ADMINISTRATION ON RAT INTESTINAL PERMEABILITY - THE ROLE OF BACTERIAL ADHERENCE
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DOI：
10.1016/s0016-5085(94)94155-6
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发表时间：
1994-01-01
期刊：
影响因子：
29.4
通讯作者：
ALVERDY, J
中科院分区：
文献类型：
--
作者：
SPITZ, J;HECHT, G;ALVERDY, J
背景/目的以前的研究表明，地塞米松给药大鼠的结果减少胆汁分泌免疫球蛋白A的浓度和改变肠道屏障功能的细菌。本研究的目的是检查和表征地塞米松对肠通透性的影响，并确定在此过程中细菌粘附于粘膜的可能影响。并在不同肠段测定地塞米松给药和肠道去污对细菌粘附和肠道通透性的影响。粘膜细菌粘附显著增加，这在盲肠中最明显（对照组为4.33 ± 0.2个菌落形成单位（cfu）（log 10）/克粘膜，地塞米松组为6.23 ± 0.2）。这伴随着肠通透性的显著改变，通过跨上皮电阻的降低来测量（对照组为49.5 ± 4.1 Ω ·cm 2，地塞米松组为25.4 ± 2.5 Ω ·cm 2）。双钠-甘露醇通量分析表明，这种缺陷主要是细胞旁的。抗生素去污的肠道完全废除了在这个model.ConclusionsBacterial-mucosal细胞的相互作用可能是负责地塞米松给药后的肠道通透性的改变观察到的肠道通透性缺陷。
Background/AimsPrevious studies have shown that dexamethasone administration to rats results in diminution of biliary secretory immunoglobulin A concentration and alters intestinal barrier function to bacteria. The aims of this study were to examine and characterize the effect of dexamethasone on intestinal permeability and to determine the possible influence of bacterial adherence to the mucosa in this process.MethodsGroups of adult Fisher rats were studied, and the effects of dexamethasone administration and bowel decontamination on bacterial adherence and intestinal permeability were determined in various bowel segments.ResultsDexamethasone administration was associated with a significant increase in bacterial adherence to the mucosa, which was most pronounced in the cecum (4.33 ± 0.2 colony forming units (cfu) (log10) per gram of mucosa for control vs. 6.23 ± 0.2 for dexamethasone). This was accompanied by significant alterations in intestinal permeability as measured by a decrease in transepithelial electrical resistance (49.5 ± 4.1 Ω · cm2for control vs. 25.4 ± 2.5 Ω · cm2for dexamethasone). Dual sodium-mannitol flux analysis showed this defect to be predominantly paracellular. Antibiotic decontamination of the intestine completely abrogated the intestinal permeability defect observed in this model.ConclusionsBacterial-mucosal cell interactions may be responsible for alterations in intestinal permeability after dexamethasone administration.