Dietary fatty acids influence sperm quality and function
Dietary fatty acids influence sperm quality and function
复制标题
膳食脂肪酸影响精子质量和功能
DOI：
10.1111/andr.12348
复制
发表时间：
2017-05-01
期刊：
影响因子：
4.5
通讯作者：
Zara, V.
中科院分区：
文献类型：
--
作者：
Ferramosca, A.;Moscatelli, N.;Zara, V.
最近，肥胖与男性不育有关。在动物模型中，高脂肪饮食通过损害配子能量代谢导致精子质量下降。本研究的目的是研究膳食脂肪酸补充剂在调节精子能量代谢中的可能作用，进而改善高脂饮食大鼠的精子质量。将性成熟的雄性Sprague-Dawley大鼠分为4组，分别饲喂标准饮食（对照组）、高脂饮食（富含35%脂肪和15%蔗糖）、补充2.5%橄榄油（单不饱和脂肪酸来源）的高脂饮食或补充2.5%磷虾油（n-3多不饱和脂肪酸来源）的高脂饮食4周。测定肝脏和脂肪组织重量、血浆葡萄糖、胰岛素和脂质浓度。分光光度法测定精子能量代谢相关酶的活性。同时测定精子线粒体呼吸效率。所获得的结果表明，橄榄油部分抵消了高脂肪饮食对精子质量的负面影响，通过增加配子活力，减少氧化应激和轻微改善线粒体呼吸效率。另一方面，磷虾油决定了精子浓度和活力的增加，乳酸脱氢酶，克雷布斯循环酶和呼吸链复合物活性的增加;还观察到细胞ATP水平的平行增加和氧化损伤的减少。这些结果表明，膳食脂肪酸能够积极影响精子质量和功能。
Recently, obesity has been linked to male infertility. In animal models the administration of a high-fat diet caused a reduction in sperm quality, by impairing gamete energy metabolism. The aim of this study was to investigate a possible effect of dietary fatty acids supplementation in the modulation of sperm energy metabolism and, in turn, in the improvement of sperm quality in rats fed a high-fat diet. Sexually mature male Sprague-Dawley rats were divided into four groups and fed for 4weeks a standard diet (control group), a high-fat diet (enriched in 35% of fat and 15% sucrose), a high-fat diet supplemented with 2.5% olive oil (a source of monounsaturated fatty acids) or a high-fat diet supplemented with 2.5% krill oil (a source of n-3 polyunsaturated fatty acids). Liver and adipose tissue weight, plasma glucose, insulin and lipid concentrations were determined. Activities of enzymes involved in sperm energetic metabolism were evaluated by spectrophotometric assays. Sperm mitochondrial respiratory efficiency was also assayed. The obtained results suggest that olive oil partially counteracts the negative effects of a high-fat diet on sperm quality, by increasing gamete motility, by reducing oxidative stress and slightly improving mitochondrial respiration efficiency. On the other hand, krill oil determines an increase in sperm concentration and motility, an increase in the activities of lactate dehydrogenase, Krebs cycle enzymes and respiratory chain complexes; a parallel increase in the cellular levels of ATP and a reduction in oxidative damage were also observed. These results suggest that dietary fatty acids are able to positively influence sperm quality and function.