New software analyses increase the reliability of measurements of retinal arterioles morphology by scanning laser Doppler flowmetry in humans
New software analyses increase the reliability of measurements of retinal arterioles morphology by scanning laser Doppler flowmetry in humans
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DOI：
10.1097/hjh.0b013e328343c27a
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发表时间：
2011-04-01
影响因子：
4.9
通讯作者：
Schmieder, Roland E.
中科院分区：
文献类型：
--
作者：
Harazny, Joanna M.;Raff, Ulrike;Schmieder, Roland E.
目的通过对视网膜小动脉的研究，为分析高血压患者体内小动脉重构提供了难得的机会。方法在重测可靠性研究中，采用Heidelberg视网膜血流计，在常规实验室条件下，对10名健康人的10只眼进行了5天的视网膜小动脉形态测量。在第二项研究中，通过三个阅读器对18名患有三种类型动脉高血压的患者的视网膜图像进行了观察者间和观察者内的可靠性分析，其中最有经验的阅读器在两个不同的日期分析了所有图像两次。通过旧的和新开发的软件版本对图像进行分析。结果经重测分析，视网膜小动脉的管腔直径、壁厚、管腔比和新计算的参数管腔比的变异系数均小于10%，但管壁横截面积的变异系数为12.5%。观察者间和观察者内的可靠性在几乎所有情况下的变异系数都小于10%，并且在不同读者和患者组之间没有差异。结论新软件SLDF Version 4.0明显提高了评估视网膜小动脉结构参数的可靠性。该应用程序提供了人体体内视网膜小动脉结构的可靠测量。J Hyperten 29：777-782(C)2011 Wolters Kluwer Health垂直酒吧Lippincott Williams&Wilkins。
Objective The investigation of the retinal arterioles offers the unique opportunity to analyze in vivo arteriolar remodeling in arterial hypertension in humans. We analyzed the reliability of assessing retinal arteriolar morphology with our new version of the software analyses for scanning laser Doppler flowmetry.Method In the test-retest reliability study, 10 eyes of 10 healthy persons were measured during 5 days under routine laboratory conditions with the Heidelberg Retinal Flowmetry. In a second study, interobserver and intraobserver reliability was analyzed from retinal images of 18 patients with three types of arterial hypertension by three readers and the most experienced reader analyzed all images twice on two different days. Images were analyzed by the old and the newly developed software versions. To characterize the reliability, the coefficients of variation were calculated.Results The test-retest study analyzed with the new program showed that the variation coefficients of vessel and lumen diameter, wall thickness, wall/lumen ratio and new calculated parameter: lumen/vessel diameter ratio of retinal arterioles were significantly less than 10%, with the exception of the wall cross-sectional area (12.5%). The interobserver and intraobserver reliability showed in nearly all circumstances coefficients of variations of less than 10% and did not differ across various readers and patient groups.Conclusion The new software 'SLDF version 4.0' clearly improved the reliability of assessing the structural parameters of the retinal arterioles. The application delivers reliable measurements of the retinal arteriolar structure in vivo in humans. J Hypertens 29:777-782 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.