The impact of Zika virus infection on human neuroblastoma (SH-SY5Y) cell line
The impact of Zika virus infection on human neuroblastoma (SH-SY5Y) cell line
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DOI：
10.4103/0972-9062.217611
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发表时间：
2017-09-01
影响因子：
0.5
通讯作者：
Poovorawan, Yong
中科院分区：
文献类型：
--
作者：
Luplertlop, Natthanej;Suwanmanee, San;Poovorawan, Yong
背景和目标：过去十年中寨卡病毒（ZIKV）流行的增加已成为全球关注的主要问题，因为该病毒同时影响新生儿和成年人。早期研究已经表明寨卡病毒感染对人类胎儿发育的影响。然而，目前还没有用于研究成人神经元 ZIKV 感染的有效体外靶细胞模型。本研究旨在建立利用人神经母细胞瘤细胞系（SH-SY5Y）来研究 ZIKV 体外感染的方法。方法：在 SH-SY5Y 细胞体外感染 ZIKV 后测定 ZIKV 生长动力学、病毒毒性和 SH-SY5Y 细胞活力。对 ZIKV 感染的 SH-SY5Y 细胞进行形态学分析，并与非人灵长类动物 Vero 细胞进行比较。此外，还测定了SH-SY5Y细胞对ZIKV感染的敏感性。结果：结果表明，ZIKV在体外能有效感染SH-SY5Y细胞系。与模拟处理或非人灵长类动物细胞相比，在 ZIKV 感染的 SH-SY5Y 细胞中观察到一些细胞稳态参数的逐渐变化，包括细胞活力、细胞毒性和细胞形态。有趣的是，在受感染的SH-SY5Y细胞的核质区中检测到了ZIKV颗粒。解释与结论：结果表明，除了已知的病毒复制细胞质区之外，在受感染的SH-SY5Y细胞的核质区中还可以检测到ZIKV颗粒。因此，SH-SY5Y细胞可以用作体外成人神经元细胞模型，以进一步阐明ZIKV生物学，并强调寨卡病毒通过核定位分布的其他可能意义，这可能与感染ZIKV的成人的神经病理学缺陷相关。
Background & objectives: An increase in Zika virus (ZIKV) epidemic during the last decade has become a major global concern as the virus affects both newborns and adult humans. Earlier studies have shown the impact of ZIKV infection in developing human foetus. However, effective in vitro model of target cells for studying the ZIKV infection in adult human neurons is not available. This study aimed to establish the use of human neuroblastoma cell line (SH-SY5Y) for studying an infection of ZIKV in vitro.Methods: ZIKV growth kinetics, viral toxicity, and SH-SY5Y cell vialibity were determined after ZIKV infection in SH-SY5Y cells in vitro. ZIKV-infected SH-SY5Y cells were morphologically analysed and compared with non-human primate Vero cells. Furthermore, the susceptibility of SH-SY5Y cells to ZIKV infection was also determined.Results: The results showed that ZIKV efficiently infects SH-SY5Y cell lines in vitro. Gradual changes of several cellular homeostasis parameters including cell viability, cytotoxicity, and cell morphology were observed in ZIKV-infected SH-SY5Y cells when compared to mock-treated or non-human primate cells. Interestingly, ZIKV particles were detected in the nucleoplasmic compartment of the infected SH-SY5Y cells.Interpretation & conclusion: The results suggest that ZIKV particle can be detected in the nucleoplasmic compartment of the infected SH-SY5Y cells beside the known viral replicating cytoplasmic area. Hence, SH-SY5Y cells can be used as an in vitro adult human neuronal cell-based model, for further elucidating the ZIKV biology, and highlight other possible significance of Zika virus distribution through nuclear localization, which may correlate to the neuropathological defects in ZIKV-infected adult humans.