AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
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DOI：
10.1186/s13059-019-1783-3
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发表时间：
2019-08-15
期刊：
影响因子：
12.3
通讯作者：
Pollard, Katherine S.
中科院分区：
文献类型：
--
作者：
Keough, Kathleen C.;Lyalina, Svetlana;Pollard, Katherine S.
CRISPR/Cas系统是一种高度特异性的基因组编辑工具，能够区分甚至单个碱基对不同的等位基因。靶位点可能携带的遗传变异无法通过基于一个参考基因组的sgRNA设计工具进行区分。AlleleAnalyzer是一个开源软件，结合单核苷酸变体和短插入和删除来设计sgrna，用于精确编辑测序基因组的1个或多个单倍型，目前支持11种Cas蛋白。它还利用共享遗传变异模式来优化不同人群的sgRNA设计。AlleleAnalyzer可在。
The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at .