Development and Optimisation of Microprojectile Systems for Plant Genetic Transformation
Development and Optimisation of Microprojectile Systems for Plant Genetic Transformation
复制标题
植物遗传转化微弹系统的开发和优化
DOI：
10.1071/pp9910453
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
T. Franks
中科院分区：
文献类型：
--
作者：
R. Birch;T. Franks
最近开发的用于将基因转移至完整细胞的微粒方法已成功地用于转化植物物种，包括一些先前抵抗使用农杆菌和原生质体介导技术的尝试的植物物种。此外，微粒轰击已被证明是唯一适合于其他应用，包括细胞器基因组的直接转化和引入完整组织的细胞中的遗传构建体的瞬时表达的快速评估。在这里，我们描述了各种微粒加速装置和必要的步骤，以开发一个有效的微粒介导的转化系统的任何植物物种。我们强调需要优化DNA到细胞中的递送，并根据靶组织的性质、组织培养中的行为和可用的标记基因来定制产生稳定转化植物的策略。模式的共转化和共表达的基因在稳定的核转化产生的微粒，并简要介绍了其他应用程序，包括细胞器转化。我们提到的微粒介导的基因转移的应用，需要克服的技术限制，如果该方法是实现其作为一个接近通用的基因转移技术，在基础植物分子生物学和实际的植物改良令人兴奋的应用程序的全部潜力。
The recently developed microprojectile method for gene transfer to intact cells has been successfully used to transform plant species including some which previously resisted attempts using Agrobacterium and protoplast mediated techniques. In addition, microprojectile bombardment has already proved uniquely suitable for other applications including direct transformation of organelle genomes and rapid assessment of transient expression of genetic constructs introduced into cells of intact tissues. Here we describe various microprojectile acceleration devices and the steps necessary to develop an effective microprojectile mediated transformation system for any plant species. We ernphasise the need to optimise the delivery of DNA into cells, and to tailor strategies for generating stably transformed plants based on the nature of the target tissue, behaviour in tissue culture, and available marker genes. Patterns of cotransformation and coexpression of introduced genes in stable nuclear transformants generated with microprojectiles are summarised, and other applications including organelle transformation are briefly described. We mention technical limitations to the application of microprojectile-mediated gene transfer which need to be overcome if the method is to achieve its full potential as a near-universal gene transfer technique with exciting applications in basic plant molecular biology and practical plant improvement.