A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
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DOI：
10.1016/j.jmb.2016.07.015
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发表时间：
2016-10-09
影响因子：
5.6
通讯作者：
Yeates, Todd O.
中科院分区：
文献类型：
--
作者：
Wheatley, Nicole M.;Eden, Kevin D.;Ngo, Joanna;Rosinski, Justin S.;Sawaya, Michael R.;Cascio, Duilio;Collazo, Michael;Hoveida, Hamidreza;Hubbell, Wayne L.;Yeates, Todd O.
自养细菌依靠各种机制来增加细胞内无机形式碳（即碳酸氢盐和二氧化碳）的浓度，以提高它们转化为有机形式的效率。跨膜碳酸氢盐转运蛋白和羧基体在碳酸氢盐和二氧化碳的积累中发挥着关键作用，但细菌中碳浓度机制的其他调节元件知之甚少。在这项研究中，在分析了 α 型羧基体操纵子周围的基因组区域后，我们描述了一种在这些操纵子中保守但之前尚未研究过的蛋白质。基于一系列脱辅基和配体结合的晶体结构和支持的生化数据，我们表明这种蛋白质，我们称之为羧基体相关PII蛋白（CPII），代表了先前研究的PII调节蛋白的广泛超家族中的一个新的、独特的亚家族，这些蛋白通常参与调节细菌中的氮代谢。 CPII 响应 ADP 结合而发生显着的构象变化，并且碳酸氢盐的存在大大增强了与核苷酸结合的亲和力。因此，CPII 似乎是一种独特类型的 PII 蛋白，可以感知碳酸氢盐的可用性，这与其与羧基体及其成分的明显基因组关联一致。
Autotrophic bacteria rely on various mechanisms to increase intracellular concentrations of inorganic forms of carbon (i.e. bicarbonate and CO2) in order to improve the efficiency with which they can be converted to organic forms. Transmembrane bicarbonate transporters and carboxysomes play key roles in accumulating bicarbonate and carbon dioxide, but other regulatory elements of carbon concentration mechanisms in bacteria are less understood. In this study, after analyzing the genomic regions around α-type carboxysome operons, we characterize a protein that is conserved across these operons but which has not been previously studied. On the basis of a series of apo- and ligand-bound crystal structures and supporting biochemical data, we show that this protein, which we refer to as the carboxysome-associated PII protein (CPII), represents a new and distinct subfamily within the broad superfamily of previously studied PII regulatory proteins, which are generally involved in regulating nitrogen metabolism in bacteria. CPII undergoes dramatic conformational changes in response to ADP binding, and the affinity for nucleotide binding is strongly enhanced by the presence of bicarbonate. CPII therefore appears to be a unique type of PII protein that senses bicarbonate availability, consistent with its apparent genomic association with the carboxysome and its constituents.
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影响因子：
14.8
作者：
Doucette, Christopher D.;Schwab, David J.;Wingreen, Ned S.;Rabinowitz, Joshua D.
通讯作者：
Rabinowitz, Joshua D.
DOI：
10.1073/pnas.0910097107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Bourrellier, Ana Belen Feria;Valot, Benoit;Hodges, Michael
通讯作者：
Hodges, Michael
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1073/pnas.0610348104
发表时间：
2007-01-23
影响因子：
11.1
作者：
Conroy, Matthew J.;Durand, Anne;Merrick, Mike
通讯作者：
Merrick, Mike
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH