THE DROSOPHILA FGF-R HOMOLOG IS EXPRESSED IN THE EMBRYONIC TRACHEAL SYSTEM AND APPEARS TO BE REQUIRED FOR DIRECTED TRACHEAL CELL EXTENSION
THE DROSOPHILA FGF-R HOMOLOG IS EXPRESSED IN THE EMBRYONIC TRACHEAL SYSTEM AND APPEARS TO BE REQUIRED FOR DIRECTED TRACHEAL CELL EXTENSION
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DOI：
10.1101/gad.5.4.697
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发表时间：
1991-04-01
影响因子：
10.5
通讯作者：
SHILO, BZ
中科院分区：
文献类型：
--
作者：
GLAZER, L;SHILO, BZ
通过低严格杂交分离出脊椎动物成纤维细胞生长因子受体（FGF-R）的果蝇同源物。 与脊椎动物中受体酪氨酸激酶的这一亚类的多样性相反，果蝇基因组似乎仅编码单一同源性。 核苷酸序列分析表明，果蝇FGF-R同源物（DFGF-R）蛋白具有保守的序列，大小和组织。 细胞外区域编码三个免疫球蛋白样结构域，并且细胞质激酶结构域表现出与脊椎动物FGF-R的高度相似性，具有典型的分裂激酶和大小不一的质膜和羧基末端区域。 DFGF-R被定位在第三条染色体上的70 C位，并鉴定出两个重叠的染色体缺陷，从而去除了该基因。 发育北方印迹显示，该基因有一个4.3 kb的单一转录本，并在发育的各个阶段表达。 转录本和蛋白质在胚胎中的定位表明，该基因主要在一组有限的组织中表达：发育中的气管系统和分层的中线神经胶质细胞和神经细胞。 在包括DFGF-R基因座在内的几个基因缺失的纯合子胚胎中，气管小凹的初始形成不受影响。 然而，导致精细树状结构形成的气管细胞突起的延伸受阻。 因此，DFGF-R蛋白可能参与接收引导气管细胞生长的空间线索。
The Drosophila homolog of the vertebrate fibroblast growth factor receptor (FGF-R) was isolated by low-stringency hybridization. In contrast to the diversity of this subclass of receptor tyrosine kinases in vertebrates, the Drosophila genome appears to encode only a single homology. Nucleotide sequence analysis demonstrates that the Drosophila FGF-R homolog (DFGF-R) protein has a conserved sequence, size, and organization. The extracellular region encodes three immunoglobulin-like domains, and the cytoplasmic kinase domain exhibits a high degree of similarity to the vertebrate FGF-Rs with the typical split kinase and comparably sized juxtamembrane and carboxy-terminal regions. The DFGF-R was mapped to position 70C on the third chromosome, and two overlapping chromosomal deficiencies that remove the gene were identified. Developmental Northern blots show that the gene has a single transcript of 4.3 kb and is expressed at all stages of development. Localization of the transcript and protein in embryos has shown that the gene is predominantly expressed in a restricted set of tissues: the developing tracheal system and the delaminating midline glial and neural cells. In embryos homozygous for a deletion of several genes including the DFGF-R locus, the initial formation of the tracheal pits is not affected. However, the extension of tracheal cell processes leading to the formation of the elaborate tree structure is blocked. The DFGF-R protein may thus participate in receiving spatial cues that guide tracheal cell outgrowth.