Structural alterations of faecal and mucosa-associated bacterial communities in irritable bowel syndrome
Structural alterations of faecal and mucosa-associated bacterial communities in irritable bowel syndrome
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DOI：
10.1111/j.1758-2229.2012.00327.x
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Moya, Andres
中科院分区：
文献类型：
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作者：
Durban, Ana;Abellan, Juan J.;Moya, Andres
肠易激综合征(IBS)是西方国家最常见的功能性胃肠道疾病。以前对IBS的研究大多基于粪便样本，表明肠道微生物区系发生了变化。然而，关于特定细菌与IBS之间的联系还没有达成共识。我们通过对扩增的16S rRNA基因进行大量测序，探索了IBS肠道细菌群落的变化。本文分析了16例IBS患者和9例健康对照的升、降结肠和粪便的粘膜活检结果。在患者和对照组的细菌群落组成中都观察到了强烈的个体间差异。这些群落在IBS病例中表现出较少的多样性。活检组织和粪便之间的微生物区系组成比患者和对照组之间的差异更大。与对照相比，我们在IBS病例中发现了一些过高和过低的分类群。检测到的变化因地点不同而不同，样本类型之间没有一致的变化。
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder in western countries. Previous studies on IBS, mostly based on faecal samples, suggest alterations in the intestinal microbiota. However, no consensus has been reached regarding the association between specific bacteria and IBS. We explore the alterations of intestinal bacterial communities in IBS using massive sequencing of amplified 16S rRNA genes. Mucosal biopsies of the ascending and descending colon and faeces from 16 IBS patients and 9 healthy controls were analysed. Strong inter-individual variation was observed in the composition of the bacterial communities in both patients and controls. These communities showed less diversity in IBS cases. There were larger differences in the microbiota composition between biopsies and faeces than between patients and controls. We found a few over-represented and under-represented taxa in IBS cases with respect to controls. The detected alterations varied by site, with no changes being consistent across sample types.