Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate
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单一大肠杆菌 ST167 临床分离株中新型 blaNDM-5 携带 IncFII 质粒和 mcr-1 携带 IncI2 质粒的表征
DOI：
10.2147/idr.s192998
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发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Xie, Weihong
中科院分区：
文献类型：
--
作者：
Xu, Lijuan;Wang, Ping;Xie, Weihong
背景和目的：质粒介导的粘菌素耐药基因mcr-1传播到新德里产金属β-内酰胺酶（NDM）的细菌分离株中可导致无法治疗的感染。在本研究中，我们对一个新的接合性bla（NDM-5）阳性IncFII质粒（pNDM-EC 16 -50）和一个携带mcr-1的IncI 2质粒在单个大肠杆菌ST 167临床分离株EC 16 - 50中进行了分子鉴定。大肠杆菌菌株ST 167和大肠杆菌A群共产NDM-5和MCR-1。S1-PFGE和Southern杂交结果表明，bla（NDM-5）和mcr-1基因分别位于近似95 kb和近似65 kb的质粒上。接合试验显示，携带bla（NDM-5）和mcr-1的质粒均为自身传播性质粒。比较质粒分析表明，携带bla（NDM-5）的F2：A-：B质粒可能是由已报道的携带NDM的pMC-NDM-like质粒通过与本地出现的pSJ_94质粒重组而进化而来的，而携带mcr-1的IncI 2质粒则与国内报道的携带mcr-1的质粒相似。大肠杆菌分离株。更引人注目的是mcr-1在成功流行的产NDM-5的E. coli克隆ST 167，该克隆可促进产碳青霉烯酶大肠杆菌中粘菌素耐药性的传播。大肠杆菌分离株。
Background and purpose: The spread of the plasmid-mediated, colistin-resistance gene mcr-1 into New Delhi metallo-beta-lactamase (NDM)-producing bacterial isolates can cause untreatable infections. In this study, we conducted a molecular characterization of a novel, conjugative, bla(NDM-5)-positive IncFII plasmid (pNDM-EC16-50) together with an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate EC16-50.Methods and results: EC16-50, which belongs to the E. coli strain ST167 and phylogroup A, was identified to co-produce NDM-5 and MCR-1. S1-PFGE and Southern blotting showed that bla(NDM-5) and mcr-1 genes were located on similar to 95 kb and similar to 65 kb plasmids, respectively. A conjugation assay revealed that both bla(NDM-5)- and mcr-1-bearing plasmids were self-transmissible. Comparative plasmid analysis suggested that bla(NDM-5)-harboring F2:A-:B-plasmid might have evolved from the well-reported NDM-carrying pMC-NDM-like plasmid via recombination with a locally emerged pSJ_94 plasmid, whereas the mcr-1-carrying IncI2 plasmid was similar to previously reported mcr-1-bearing plasmids in China.Conclusion and impact: This study represents the first report of the NDM-5 carrying Inc-FII- but not IncX3-type plasmid in an MCR-1-producing E. coli isolate. More striking was the dissemination of mcr-1 in a successful epidemic NDM-5-producing E. coli clone ST167, which could facilitate the spread of colistin resistance in carbapenemase-producing E. coli isolates.