Collagen-coated polycaprolactone microparticles as a controlled drug delivery system
Collagen-coated polycaprolactone microparticles as a controlled drug delivery system
复制标题
DOI：
10.1080/02652040801972004
复制
发表时间：
2008-01-01
影响因子：
3.9
通讯作者：
Sehgal, Praveen Kumar
中科院分区：
文献类型：
--
作者：
Aishwarya, S.;Mahalakshmi, S.;Sehgal, Praveen Kumar
目的：评估乙酰化胶原蛋白包被的聚己内酯（PCL）微粒作为控释药物递送系统的用途。方法：研究了 PCL 微粒和含有盐酸多西环素（DH）的胶原蛋白包被的 PCL 微粒的表面形态、药物封装和释放曲线，以开发一种控释系统，该系统还可作为细胞附着的支架。采用乳液溶剂蒸发技术制备PCL微粒，并负载DH。由于包封率较低，PCL 微粒被含有 DH 的乙酰化胶原蛋白包被，以提高药物利用率。通过乙酰化修饰胶原蛋白以改变其等电点并获得pH 7.0的乙酰化胶原蛋白溶液。使用扫描电子显微镜 (SEM) 对微粒进行表征，并使用 HPLC 测定体外药物释放曲线。结果：在外水相中使用 4% PVA 制备了尺寸均匀（接近 1000 nm）的 PCL 微粒。将 pH 7.0 的乙酰化胶原蛋白涂覆到 PCL 微粒上。这导致在中性pH下产生尺寸均匀的微粒。 PCL 充当胶原蛋白的支持物，胶原蛋白充当细胞附着的支架。体外药物释放研究表明，胶原蛋白包被的 PCL 微粒是释放持续时间超过 10 天的受控药物递送系统的有希望的候选者。体外成纤维细胞培养研究表明，胶原蛋白是细胞附着的良好基质，可为细胞增殖和再生提供稳定的环境。因此，该系统对于短期药物输送来说是理想的，以创造一个无菌环境，使细胞可以粘附和增殖以再生该部位。
Objective: Polycaprolactone (PCL) microparticles coated with acetylated collagen have been assessed for use as a controlled drug delivery system.Method: The surface morphology, drug encapsulation and release profile of PCL microparticles and collagen-coated PCL microparticles containing doxycycline hydrochloride (DH) have been investigated in order to develop a controlled release system which would in addition act as a scaffold for cell attachment. PCL microparticles were prepared by emulsion solvent evaporation technique and loaded with DH. Since the encapsulation was found to be low, PCL microparticles were coated with acetylated collagen containing DH, to increase the drug availability. Collagen was modified by acetylation to shift its isoelectric point and to have acetylated collagen solution at pH 7.0. The microparticles were characterized using a scanning electron microscope (SEM) and the in vitro drug release profile was determined using HPLC.Results: Uniform sized (similar to 1000 nm) PCL microparticles were prepared using 4% PVA in the external water phase. Acetylated collagen at pH 7.0 was coated onto the PCL microparticles. This resulted in microparticles of uniform size at neutral pH. PCL acts as a support for collagen which acts as a scaffold for cell attachment. In vitro drug release studies show that collagen-coated PCL microparticle is a promising candidate for controlled drug delivery system having release duration of over 10 days. In vitro fibroblast culture studies reveal that collagen is a good substrate for cell attachment and would provide a stable environment for cell proliferation and regeneration. Thus, this system would be ideal for a short-term drug delivery to create an aseptic environment where cells can adhere and proliferate to regenerate the site.