Human chorionic gonadotropin increases chromatin solubility in isolated bovine and human luteal nuclei.
Human chorionic gonadotropin increases chromatin solubility in isolated bovine and human luteal nuclei.
复制标题
人绒毛膜促性腺激素增加分离的牛和人黄体核中染色质的溶解度。
DOI：
10.1095/biolreprod41.4.753
复制
发表时间：
1989
影响因子：
3.6
通讯作者：
Chegini,N
中科院分区：
文献类型：
--
作者：
Oechsli,M;Rao,CV;Chegini,N
We have previously demonstrated that bovine and human luteal nuclei contain human chorionic gonadotropin/luteinizing hormone (hCG/LH) receptors and that these gonadotropins can directly stimulate nuclear membrane enzyme activity (nucleoside triphosphatase) involved in messenger ribonucleic acid (mRNA) transport from the nucleus to the cytoplasm. The present studies were undertaken to investigate the effect or hCG on chromatin solubility, reflecting perhaps synthesis and transport of RNA, in isolated bovine and human luteal nuclei. hCG increased chromatin solubility in a concentration-dependent manner. This hCG effect is either blocked or substantially reduced by the addition of hCG antiserum; denatured hCG had no effect and cyclic adenosine 3′,5′-monophosphate could not mimic the hCG response. hCG had no effect on chromatin solubility in bovine liver or kidney nuclei and hormones other than hCG, human LH, or the β subunit of hCG had no effect on chromatin solubility in bovine luteal nuclei, demonstrating the tissue and hormone specificity of the response. These findings further strengthen the concept of direct gonadotropin regulation of nuclear functions of luteal cells.
