Transcriptional regulation of Salmonella virulence: A phoQ periplasmic domain mutation results in increased net phosphotransfer to phoP
Transcriptional regulation of Salmonella virulence: A phoQ periplasmic domain mutation results in increased net phosphotransfer to phoP
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DOI：
10.1128/jb.178.21.6369-6373.1996
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发表时间：
1996-11-01
影响因子：
3.2
通讯作者：
Miller, SI
中科院分区：
文献类型：
--
作者：
Gunn, JS;Hohmann, EL;Miller, SI
phoP/phoQ 位点 (pho-24) 中的突变导致 PhoP 激活基因（表型 PhoP 组成型 [PhoP(c)]）的表达不受调节，被映射到 phoQ。 PhoQ 48 位的 Thr 变为 Ile 是导致 PhoP(c) 表型（小鼠毒力减弱、上皮细胞侵袭缺陷和巨噬细胞宽敞吞噬体形成）的原因。膜提取物的 PhoP 磷酸化需要 PhoQ，而含有 PhoQ Ile-48 的膜显示出 PhoP 的净磷酸化增加。
A mutation in the phoP/phoQ locus (pho-24) that results in unregulated expression of PhoP-activated genes (phenotype PhoP constitutive [PhoP(c)]) was mapped to phoQ. Change of a Thr to Ile at position 48 of PhoQ was responsible for the PhoP(c) phenotype (attenuation of mouse virulence, defects in epithelial cell invasion, and macrophage spacious phagosome formation). PhoP phosphorylation by membrane extracts required PhoQ, and PhoQ Ile-48-containing membranes demonstrated increased net phosphorylation of PhoP.