Observation of the developmental pattern and morphological change of serosal cuticle in Locusta migratoria (Orthoptera: Acrididae) with an optimized paraffin section method
Observation of the developmental pattern and morphological change of serosal cuticle in Locusta migratoria (Orthoptera: Acrididae) with an optimized paraffin section method
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DOI：
10.16380/j.kcxb.2018.06.012
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发表时间：
2018-06-20
期刊：
影响因子：
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通讯作者：
Zhang Ting-Ting
中科院分区：
文献类型：
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作者：
Liu Wei-Wei;Li Ren-Jian;Zhang Ting-Ting
[Aim]本研究旨在优化东亚飞蝗（Locusta migratoria）胚胎早期的石蜡切片技术，并探讨其角质层的发育规律。[方法]采用石蜡切片技术，对L.在固定、洗涤、脱水、澄清等步骤上对飞蝗胚胎进行了改进。采用石蜡切片技术，对3-10天胚胎的角质层进行HE染色和荧光染色，分析角质层的发育过程。移民。用石蜡切片HE染色法观察了卵孔至尾的胚胎发育过程。从第6-8天的胚胎中，将其纵向切割。[结果]优化了L.飞蝗胚胎包括：将鸡蛋用NaClO预处理，固定前打孔，然后将固定后的鸡蛋分别清洗、脱水、清洗30 min。采用我们优化的石蜡切片技术，获得了完整清晰的胚胎期表皮结构切片。在30 ℃下，在胚胎阶段，第5天完成角质层形成和几丁质沉积，第8天开始降解。在胚胎发育的第6 ~ 7天，胚胎发生退化，并伴有浆膜和浆膜角质层的形态变化。[结论]优化了L. migratoria胚的发育模式，揭示了角质层的发育模式。本研究为进一步研究罗氏沼虾胚胎发育代谢奠定了基础。迁徙者。
[Aim] The aim of this study is to optimize the paraffin section technology and to examine the developmental pattern of serosal cuticle in the early embryonic stage of Locusta migratoria. [Methods] The paraffin section technology for L. migratoria embryo was modified on the steps of fixation, washing, dehydration and clearing. The developmental process of serosal cuticle was analyzed with hematoxylineosin staining (HE staining) and fluorescent staining following paraffin section of day 3-10 embryos of L. migratoria. The katatrepsis of embryo from micropyle to tail was observed by HE staining of the paraffin sections of L. migratoria from day 6-8 embryos, which were cut longitudinally. [Results] The optimized paraffin section steps for L. migratoria embryo included: pretreating the egg with NaCIO and punching it before fixation, then washing, dehydrating and clearing the fixed egg for 30 min, respectively. Integrative and clear sections of the whole epidermis structure of the embryonic stage were obtained by using our optimized paraffin section technology. The serosal cuticle formation and chitin deposition were completed on day 5 and began to degrade on day 8 during the embryonic stages at 30 degrees C. The katatrepsis occurred from day 6 to day 7 in the embryonic stages, accompanied by the morphological change of serosa and serosal cuticle. [Conclusion] We optimized the paraffin section technology for L. migratoria embryo, and revealed the developmental pattern of serosal cuticle and katatrepsis. This study lays a foundation for studying the metabolisms of embryonic development in L. migratoria.