Screw-sense inversion characteristic of α-helical poly(β-p-chlorobenzyl L-aspartate) and comparison with other related polyaspartates
Screw-sense inversion characteristic of α-helical poly(β-p-chlorobenzyl L-aspartate) and comparison with other related polyaspartates
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DOI：
10.1002/bip.20207
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发表时间：
2005-01-01
期刊：
影响因子：
2.9
通讯作者：
Furuya, H
中科院分区：
文献类型：
--
作者：
Abe, A;Hiraga, K;Furuya, H
这是20世纪60年代古德曼及其同事的开创性工作中关于聚天冬氨酸螺旋反转的一系列研究之一。聚(β-对氯苯基(L)-天冬氨酸)(PClBLA)是目前研究较多的溶液和固态聚天冬氨酸衍生物之一。PClBLA的化学结构与聚β-苯基(L)-天冬氨酸(PBLA)和聚β-苯乙基(L)-天冬氨酸(PPLA)不同，只是在相对较长的侧链末端。在传统的螺旋溶剂中，PBLA呈左旋形式(L)，不显示任何螺旋感觉反转。与PBLA相反，PClBLA和PPLA在氯代烷烃溶剂中都形成右旋螺旋(R)，并且在较高的温度下表现出反转的α-螺旋感觉。然而，这两种聚合物在变性酸存在下的转变行为有很大的不同。当PPLA通过降温呈现R->；L->；线圈等转变时，PCLBLA直接进入线圈状态，而不显示重入L形式。通过构建相图，研究了这些聚合物之间的唯象差异的原因。(C)2005年威利期刊公司生物聚合物(Pept Sci)80：249-257,2005。
This is one of a series of studies on the reversal of the helix sense of polyaspartates originated from the pioneering work of Goodman and his associates in 1960s. Poly(beta-p-chlorobenzyl (L)-aspartate) (PClBLA) is one of the well-studied polyaspartate derivatives in both solution and the solid state. The chemical structure of PClBLA differs from those of poly(beta-benzyl (L)-aspartate) (PBLA) and poly(beta-phenethyl (L)-aspartate) (PPLA) only at the terminal of the relatively long side chain. PBLA takes a left-handed form (L) in conventional helicoidal solvents and does not exhibit any screw-sense inversion. In contrast to PBLA, both PClBLA and PPLA form a right-handed helix (R) in chlorinated alkane solvents and exhibits a reversal of a-helix sense at higher temperatures. Yet the transition behaviors in the presence of denaturant acid are quite different between these two polymers. While PPLA exhibits transitions such as R -> L -> coil by lowering temperature, PClBLA directly goes into the coil state without showing the reentrant L form. The cause of these phenomenological differences among these polymers has been investigated by constructing the phase diagram. (c) 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 80: 249-257,2005.