Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1a but not Pbx1b
Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1a but not Pbx1b
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DOI：
10.1093/emboj/17.13.3714
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发表时间：
1998-07-01
期刊：
影响因子：
11.4
通讯作者：
Sauvageau, G
中科院分区：
文献类型：
--
作者：
Kroon, E;Krosl, J;Sauvageau, G
Hoxa9， Meis1和Pbx1编码含有同源结构域的蛋白，这些蛋白与小鼠和人类的白血病转化有关。Hoxa9、Meis1和Pbx1蛋白之间存在物理相互作用，Hoxa9与Meis1和Pbx1共同结合一致的DNA序列，而Meis1和Pbx1在DNA存在和不存在的情况下都形成异源二聚体。在这项研究中，我们试图确定Hoxa9是否可以与Pbx1或Meis1合作转化造血细胞。通过逆转录病毒工程，使原代骨髓细胞同时过表达Hoxa9和Meis1a，诱导生长因子依赖性低克隆急性髓系白血病
Hoxa9, Meis1 and Pbx1 encode homeodomain-containing proteins implicated in leukemic transformation in both mice and humans. Hoxa9, Meis1 and Pbx1 proteins have been shown to physically interact with each other, as Hoxa9 cooperatively binds consensus DNA sequences with Meis1 and with Pbx1, while Meis1 and Pbx1 form heterodimers in both the presence and absence of DNA. In this study, we sought to determine if Hoxa9 could transform hemopoietic cells in collaboration with either Pbx1 or Meis1. Primary bone marrow cells, retrovirally engineered to overexpress Hoxa9 and Meis1a simultaneously, induced growth factor-dependent oligoclonal acute myeloid leukemia in