Association of the influenza a virus RNA-dependent RNA polymerase with cellular RNA polymerase II
Association of the influenza a virus RNA-dependent RNA polymerase with cellular RNA polymerase II
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DOI：
10.1128/jvi.79.9.5812-5818.2005
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发表时间：
2005-05-01
影响因子：
5.4
通讯作者：
Fodor, E
中科院分区：
文献类型：
--
作者：
Engelhardt, OG;Smith, M;Fodor, E
流感病毒RNA依赖性RNA聚合酶的转录依赖于已知与细胞RNA聚合酶II（Pol II）转录相关的细胞RNA加工活性，即加帽和剪接。因此，人们假设病毒RNA聚合酶和Pol II的转录可能在功能上存在联系。在这里，我们首次证明了流感病毒RNA聚合酶复合物通过其C-末端结构域与Pol II的大亚基相互作用。病毒聚合酶结合Pol II的过度磷酸化形式，表明其靶向积极转录Pol II。此外，免疫荧光分析与一种新的模型一致，该模型显示流感病毒聚合酶在Pol II转录位点积累。目前的研究结果提供了一个框架，进一步研究，以阐明转录的病毒RNA聚合酶的机制原则，并在感染细胞中的Pol II活性的调节有影响。
Transcription by the influenza virus RNA-dependent RNA polymerase is dependent on cellular RNA processing activities that are known to be associated with cellular RNA polymerase II (Pol II) transcription, namely, capping and splicing. Therefore, it had been hypothesized that transcription by the viral RNA polymerase and Pol II might be functionally linked. Here, we demonstrate for the first time that the influenza virus RNA polymerase complex interacts with the large subunit of Pol II via its C-terminal domain. The viral polymerase binds hyperphosphorylated forms of Pol II, indicating that it targets actively transcribing Pol II. In addition, immunofluorescence analysis is consistent with a new model showing that influenza virus polymerase accumulates at Pol II transcription sites. The present findings provide a framework for further studies to elucidate the mechanistic principles of transcription by a viral RNA polymerase and have implications for the regulation of Pol II activities in infected cells.