Single anion-selective channels in basolateral membrane of a mammalian tight epithelium.
Single anion-selective channels in basolateral membrane of a mammalian tight epithelium.
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哺乳动物紧密上皮基底外侧膜中的单一阴离子选择性通道。
DOI：
10.1073/pnas.82.22.7791
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发表时间：
1985
影响因子：
11.1
通讯作者：
Lewis,SA
中科院分区：
文献类型：
--
作者：
Hanrahan,JW;Alles,WP;Lewis,SA
应用膜片钳技术研究了兔膀胱上皮细胞基底外侧膜氯离子通透性。观察到两种类型的阴离子选择性通道。一种通道类型表现为内向整流，并在-50 mV的电导为64 pS时，对称地沐浴含150 mM氯化物的盐溶液;其他类似于高电导电压依赖性阴离子通道（VDACs）。两个通道的选择性顺序为Cl-约等于Br-约等于I-约等于SCN-约等于NO3-大于F-大于乙酸盐大于葡萄糖酸盐大于Na+约等于K+，并且对阴离子交换抑制剂4，4 '-二异硫氰基芪-2，2'-二磺酸敏感。膀胱中的基底侧氯电导显然是由于64 pS阴离子通道，其在生理电位下是活跃的。该通道对阳离子的不完美的选择性也可能解释了基底外侧膜的低但有限的钠渗透性。
Basolateral membrane chloride permeability of surface cells from rabbit urinary bladder epithelium was studied using the patch-clamp technique. Two types of anion-selective channel were observed. One channel type showed inward rectification and had a conductance of 64 pS at-50 mV when bathed symmetrically by saline solution containing 150 mM chloride; the other resembled high-conductance voltage-dependent anion channels (VDACs). Both channels had the selectivity sequence Cl-approximately equal to Br-approximately equal to I- approximately equal to SCN- approximately equal to NO3- greater than F- greater than acetate greater than gluconate greater than Na+ approximately equal to K+ and were sensitive to the anion exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Basolateral chloride conductance in urinary bladder is apparently due to the 64 pS anion channel, which is active at physiological potentials. Imperfect selectivity of this channel against cations might also account for the low, but finite, sodium permeability of the basolateral membrane.