Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
复制标题
将 DNA、RNA 和蛋白质电泳转移到重氮苄氧基甲基 (DBM) 纸上。
DOI：
10.1093/nar/8.2.299
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发表时间：
1980
影响因子：
14.9
通讯作者：
Dahlberg,AE
中科院分区：
文献类型：
--
作者：
Stellwag,EJ;Dahlberg,AE
本文报道了一种将DNA、RNA、蛋白质和核糖核蛋白颗粒从各种凝胶电泳转移到二芳苄氧基甲基（DBM）纸上的方法。这些大分子的电泳转移条件已被优化，以允许几乎定量的转移和共价偶联。DNA和RNA经电泳转移到DBM-纸上，保留了它们与特异性探针杂交的能力。DBM-纸的高转移效率和核酸的高容量使得特异性核苷酸序列的灵敏检测成为可能。在蛋白质到DBM纸的电泳转移和共价偶联中实现了类似的效率。大分子也可以被电泳转移并结合到不能形成共价键的DBM纸上。它们在高盐条件下从纸上洗脱下来，为DNA、RNA和蛋白质的分离提供了一种新的、有用的制备方法。
A method has been developed for the electrophoretic transfer of DNA,RNA,protein and ribonucleoprotein particles from a variety of gels onto diarobenryloxymethyl (DBM) paper. Conditions for the electrophoretic transfer of these macromolecules have been optimized to allow for nearly quantitative transfer and covalent coupling. DNA and RNA electrophoretically transferred to DBM-paper retain their ability to hybridize with specific probes. The high efficiency of transfer and the high capacity of DBM-paper for nucleic acids makes possible the sensitive detection of specific nucleotide sequences. Similar efficiency is achieved in electrophoretic transfer and covalent coupling of proteins to DBM-paper. Macromolecules can also be electrophoretically transferred and bound to DBM-paper incapable of covalent bond formation. Their elution from the paper in high salt provides a new and useful preparative method for isolation of DNA,RNA and protein.