A molecular link between gene-specific and chromosome-wide transcriptional repression
A molecular link between gene-specific and chromosome-wide transcriptional repression
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DOI：
10.1101/gad.972702
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发表时间：
2002-04-01
影响因子：
10.5
通讯作者：
Meyer, BJ
中科院分区：
文献类型：
--
作者：
Chu, DS;Dawes, HE;Meyer, BJ
转录调控的基因特异性和染色体范围机制控制多细胞生物的发育。 SDC-2 是秀丽隐杆线虫雌雄同体命运的决定因素，是两种调节模式的范例。 SDC-2抑制X染色体的转录以实现剂量补偿，并且还抑制雄性性别决定基因her-1以引发雌雄同体分化。我们在此表明，SDC-2 将整个剂量补偿复合物募集到 her-1，指导这种 X 染色体抑制机制沉默个体常染色体基因。对her-1体内功能的解剖揭示了SDC-2结合、剂量补偿复合物的募集和转录抑制所需的DNA识别元件。 her-1 内的元件在位置、序列和抑制强度上有所不同，这意味着剂量补偿复合物可能使用在抑制中发挥不同作用的不同识别元件来调节沿 X 染色体的转录。
Gene-specific and chromosome-wide mechanisms of transcriptional regulation control development in multicellular organisms. SDC-2, the determinant of hermaphrodite fate in Caenorhabditis elegans, is a paradigm for both modes of regulation. SDC-2 represses transcription of X chromosomes to achieve dosage compensation, and it also represses the male sex-determination gene her-1 to elicit hermaphrodite differentiation. We show here that SDC-2 recruits the entire dosage compensation complex to her-1, directing this X-chromosome repression machinery to silence an individual, autosomal gene. Functional dissection of her-1 in vivo revealed DNA recognition elements required for SDC-2 binding, recruitment of the dosage compensation complex, and transcriptional repression. Elements within her-1 differed in location, sequence, and strength of repression, implying that the dosage compensation complex may regulate transcription along the X chromosome using diverse recognition elements that play distinct roles in repression.