Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia.
Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia.
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DOI：
10.1182/blood.v60.4.959.bloodjournal604959
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发表时间：
1982-10
期刊：
影响因子：
20.3
通讯作者：
A. Look;S. Melvin;D. Williams;G. Brodeur;G. Dahl;D. Kalwinsky;S. Murphy;A. Mauer
中科院分区：
文献类型：
--
作者：
A. Look;S. Melvin;D. Williams;G. Brodeur;G. Dahl;D. Kalwinsky;S. Murphy;A. Mauer
应用流式细胞术（FCM）检测了225例未经治疗的急性白血病患儿骨髓原始细胞碘化丙啶染色后细胞DNA含量分布，并与白血病细胞表型和核型进行了相关性分析。54例（24%）原发恶性肿瘤干细胞检测到非整倍体，其中51例为超二倍体，3例为亚二倍体。28例（23例ALL，5例ANLL）经FCM检测发现第二个干细胞系的DNA含量约为原代干细胞系的两倍，可能是白血病细胞异质性的重要来源。流式细胞术检测的DNA含量异常程度与白血病核型中整条染色体的增加或丢失数高度相关（r = 0.98）。FCM检测到的非整倍体在急性淋巴细胞白血病（ALL）中（52/173，30.1%）比急性非淋巴细胞白血病（2/52，3.8%）更常见（p <0.001）。在ALL组中，非整倍体与常见ALL抗原的细胞表面表达显着相关：127例抗原阳性病例中有46例为非整倍体，而46例抗原阴性病例中有6例为非整倍体（p小于0.003）。21例无常见ALL抗原的T细胞ALL中仅2例可检测到非整倍体，显著低于常见ALL组（p = 0.02）。B细胞和红细胞花环阳性T细胞ALL的S期细胞百分比中位数显著高于其他表型亚组。结论：异倍体和S期细胞比例与白血病细胞分化状态有关。相关性的生物学基础尚未建立，但可能与恶性转化过程有关。
Cellular DNA content distributions of propidium-iodide-stained bone marrow blasts were determined by flow cytometry (FCM) for 225 untreated children with acute leukemia and were correlated with leukemia cell phenotype and karyotype. Aneuploidy of the primary malignant stem line was detected in 54 cases (24%): 51 hyperdiploid and 3 hypodiploid. A second stem line with approximately twice the DNA content of the primary stem line was recognized by FCM in 28 cases (23 ALL, 5 ANLL) and may be an important source of leukemia cell heterogeneity. The degree of DNA content abnormality detected by FCM was highly correlated (r = 0.98) with the number of whole chromosome gains or losses in the leukemia karyotype. Aneuploidy detectable by FCM was more frequent in acute lymphoblastic leukemia (ALL) (52 of 173, 30.1%) than in acute nonlymphoblastic leukemia (2 of 52, 3.8%) (p less than 0.001). In the ALL group, aneuploidy was significantly correlated with the cell surface expression of common ALL antigen: 46 of 127 antigen-positive cases were aneuploid compared to 6 of 46 antigen-negative cases (p less than 0.003). Only 2 of 21 cases of T-cell ALL without common ALL antigen had detectable aneuploidy, which was significantly less than in the common ALL group (p = 0.02). The median percentage of cells in S-phase was significantly greater for B-cell and erythrocyte rosette-positive T-cell ALL, than for the other phenotypic subgroups. We conclude that aneuploidy and S-phase cell percentage are correlated with the state of leukemia cell differentiation. The biologic basis for the correlation is not established, but may be linked to the process of malignant transformation.