Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells
Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells
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DOI：
10.1038/sj.embor.embor799
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发表时间：
2003-04-01
期刊：
影响因子：
7.7
通讯作者：
Nyström, T
中科院分区：
文献类型：
--
作者：
Desnues, B;Cuny, C;Nyström, T
潜在的致病菌，如大肠杆菌和霍乱弧菌，在停滞期间变得不能培养。由于研究细菌群体异质性的困难，对这类细胞的分析一直受到阻碍。通过原位检测单个大肠杆菌细胞中的蛋白质氧化，并使用密度梯度离心法分离可培养细胞和非可培养细胞，我们发现非可培养细胞中的蛋白质表现出增加的和不可逆转的氧化损伤，这影响到各种细菌和蛋白质。在非可培养细胞中，特定应激调节蛋白的表达水平较高，证实了与氧化损伤有关的缺陷增加以及蛋白质异常的发生，如氨基酸误结合。我们的数据表明，不可培养的细胞是由于随机恶化而产生的，而不是一种适应性的计划，并准确地指出氧化管理是这些细胞的“阿喀琉斯之踵”。
Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells.